Interactive design and validation of antibody panels using single-cell RNA-seq atlases

Watson, M.; Latour, S.; Abazari, G.; Geuenich, M. J.; Poon, E.; Cao, R.; Mrkonjic, M.; McGuigan, A. P.; Jackson, H. W.; Campbell, K. R.

doi:10.1101/2024.10.15.618479

bioRxiv · 10.1101/2024.10.15.618479

Interactive design and validation of antibody panels using single-cell RNA-seq atlases

Watson, M.·Latour, S.·Abazari, G.·Geuenich, M. J.·Poon, E.·Cao, R.·Mrkonjic, M.·McGuigan, A. P.·Jackson, H. W.·Campbell, K. R.

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Abstract

Single-cell RNA-sequencing holds promise for identifying novel markers of cellular variation for antibody-based technologies. However, antibody panel design is often difficult due to multiple experimental and biological constraints. We introduce Cytomarker, an interactive platform enabling human-in-the-loop design of antibody panels from single-cell transcriptomic data. We use Cytomarker to spatially profile human mammary tissue subpopulations and develop a novel antibody screening approach to validate granular subpopulation predictions across >3.5M cells.

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Watson, M., Latour, S., Abazari, G., Geuenich, M. J., Poon, E., Cao, R., Mrkonjic, M., McGuigan, A. P., Jackson, H. W., Campbell, K. R.. 2024-10-18. Interactive design and validation of antibody panels using single-cell RNA-seq atlases. https://doi.org/10.1101/2024.10.15.618479

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