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bioRxiv · 10.1101/2024.10.14.617874

Dexrazoxane as a viable microsporidia control agent in Anopheles gambiae

Zeferino, T. G.·Silva, L. M.
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Abstract

Microsporidia have long been proposed as biological agents for controlling disease vectors and the parasites they transmit. However, their study in vector biology has been constrained due to challenges in manipulating microsporidia within hosts. In this study, we investigated the effect of Dexrazoxane, a candidate drug against microsporidiosis, on the establishment and development of Vavraia culicis infection in its natural host, the mosquito Anopheles gambiae, the main malaria vector. Our findings show that Dexrazoxane significantly reduces spore load, particularly in mosquitoes reared individually, without affecting the overall infection success of the parasite. This result aligns with studies in Caenorhabditis elegans, where Dexrazoxane inhibited new spore production without hindering initial spore integration into the host gut cells. Dexrazoxanes DNA topoisomerase II inhibitor mechanism likely explains its impact on mosquito development, as larvae exposed to the drug failed to emerge as adults. These findings highlight Dexrazoxanes potential as a viable tool for controlling microsporidia in adult mosquitoes and hope to enhance the study of mosquito-microsporidia interactions. Further research is required to explore its broader application in vector-borne disease control, including malaria.

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Zeferino, T. G., Silva, L. M.. 2024-10-17. Dexrazoxane as a viable microsporidia control agent in Anopheles gambiae. https://doi.org/10.1101/2024.10.14.617874

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