The C. elegans homolog of Sjögrens Syndrome Nuclear Antigen 1 is required for the structural integrity of the centriole and bipolar mitotic spindle assembly

Pfister, J. A.; Agostini, L.; Bournonville, L.; Sankaralingam, P.; Bell, Z. G.; Hamel, V.; Guichard, P.; Biertumpfel, C.; Mizuno, N.; O'Connell, K. F.

doi:10.1101/2024.10.03.616528

bioRxiv · 10.1101/2024.10.03.616528

The C. elegans homolog of Sjögrens Syndrome Nuclear Antigen 1 is required for the structural integrity of the centriole and bipolar mitotic spindle assembly

Pfister, J. A.·Agostini, L.·Bournonville, L.·Sankaralingam, P.·Bell, Z. G.·Hamel, V.·Guichard, P.·Biertumpfel, C.·Mizuno, N.·O'Connell, K. F.

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Abstract

Centrioles play central roles in ciliogenesis and mitotic spindle assembly. Once assembled, centrioles exhibit long-term stability, a property essential for maintaining numerical control. How centriole stability is achieved and how it is lost in certain biological contexts are still not completely understood. In this study we show that SSNA-1, the Caenorhabditis elegans ortholog of Sjogrens Syndrome Nuclear Antigen 1, is a centriole constituent that localizes close to the microtubule outer wall, while also exhibiting a developmentally regulated association with centriole satellite-like structures. A complete deletion of the ssna-1 gene results in an embryonic lethal phenotype marked by the appearance of extra centrioles and spindle poles. We show that SSNA-1 genetically interacts with the centriole stability factor SAS-1 and is required post assembly for centriole structural integrity. In SSNA-1s absence, centrioles assemble but fracture leading to extra spindle poles. However, if the efficiency of cartwheel assembly is reduced, the absence of SSNA-1 results in daughter centriole loss and monopolar spindle formation, indicating that the cartwheel and SSNA-1 cooperate to stabilize the centriole during assembly. Our work thus shows that SSNA-1 contributes to centriole stability during and after assembly, thereby ensuring proper centriole number.

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Pfister, J. A., Agostini, L., Bournonville, L., Sankaralingam, P., Bell, Z. G., Hamel, V., Guichard, P., Biertumpfel, C., Mizuno, N., O'Connell, K. F.. 2024-10-04. The C. elegans homolog of Sjögrens Syndrome Nuclear Antigen 1 is required for the structural integrity of the centriole and bipolar mitotic spindle assembly. https://doi.org/10.1101/2024.10.03.616528

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