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bioRxiv · 10.1101/2024.10.02.616289

Understanding genetic diversity and phylogeography of Common Teal and its phylogenetic relationship with other water bird species in the wetlands of Kashmir Himalayas.

Ahmad, K.·Bisht, D.·Joshi, B. D.·Goyal, S. P.·Nigam, P. P.·Khan, K. A.·Haq, I. U.·Javid, M.
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Abstract

Understanding the genetic diversity and phylogeography of migratory species is critical for biodiversity conservation and the effective management of wetland ecosystems. The Kashmir Himalayas, an integral part of the Central Asian Flyway, host several key wetlands that provide critical wintering grounds for a variety of migratory birds. This study focuses on assessing the genetic characteristics and phylogenetic relationships of the Common Teal (Anas crecca) in comparison to other species within the families Anatidae and Rallidae. We analysed 149 blood samples, including 71 from A. crecca and 78 from other species in the two families, collected from wetlands in the Kashmir region. Using four mitochondrial markers--cytochrome oxidase subunit I (COI), cytochrome b (Cyt b), 16S rRNA, and the control region--we evaluated the genetic diversity and lineage connectivity of these species. Our findings reveal that the mitochondrial DNA haplotypes of A. crecca in the Kashmir Himalayas are shared with European populations, indicating strong maternal gene flow and connectivity between distant populations. A minimum spanning haplotype network analysis showed minimal nucleotide differences among haplotypes, particularly in the Cyt b and control regions, suggesting low genetic differentiation and a high degree of similarity among individuals. Notably, we identified at least four distinct maternal lineages of A. crecca in the Kashmir wetlands, reflecting diverse migratory sources. Our results also highlight that DNA barcoding using COI exhibited both high and low species resolution, with significant intraspecific variation, making it a valuable tool for further phylogeographic studies. The observed genetic diversity and haplotype sharing with distant populations underscore the ecological importance of Kashmirs wetlands as crucial habitats for migratory species. Our study emphasizes the need for targeted conservation and management strategies to preserve these vital ecosystems and the biodiversity they support.

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Ahmad, K., Bisht, D., Joshi, B. D., Goyal, S. P., Nigam, P. P., Khan, K. A., Haq, I. U., Javid, M.. 2024-10-03. Understanding genetic diversity and phylogeography of Common Teal and its phylogenetic relationship with other water bird species in the wetlands of Kashmir Himalayas.. https://doi.org/10.1101/2024.10.02.616289

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