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bioRxiv · 10.1101/2024.10.01.616173

A molecular representation system with a common reference frame for natural products pathway discovery and structural diversity tasks.

Abstract

Researchers have uncovered hundreds of thousands of natural products, many of which contribute to medicine, materials, and agriculture. However, missing knowledge of the biosynthetic pathways to these products hinders their expanded use. Nucleotide sequencing is key in pathway elucidation efforts, and analyses of natural products molecular structures, though seldom discussed explicitly, also play an important role by suggesting hypothetical pathways for testing. Structural analyses are also important in drug discovery, where many molecular representation systems - methods of representing molecular structures in a computer-friendly format - have been developed. Unfortunately, pathway elucidation investigations seldom use these representation systems. This gap is likely because those systems are primarily built to document molecular connectivity and topology, rather than the absolute positions of bonds and atoms in a common reference frame, the latter of which enables chemical structures to be connected with potential underlying biosynthetic steps. Here, we present a unique molecular representation system built around a common reference frame. We tested this system using triterpenoid structures as a case study and explored the systems applications in biosynthesis and structural diversity tasks. The common reference frame system can identify structural regions of high or low variability on the scale of atoms and bonds and enable hierarchical clustering that is closely connected to underlying biosynthesis. Combined with phylogenetic distribution information, the system illuminates distinct sources of structural variability, such as different enzyme families operating in the same pathway. These characteristics outline the potential of common reference frame molecular representation systems to support large-scale pathway elucidation efforts. Significance StatementStudying natural products and their biosynthetic pathways aids in identifying, characterizing, and developing new therapeutics, materials, and biotechnologies. Analyzing chemical structures is key to understanding biosynthesis and such analyses enhance pathway elucidation efforts, but few molecular representation systems have been designed with biosynthesis in mind. This study developed a new molecular representation system using a common reference frame, identifying corresponding atoms and bonds across many chemical structures. This system revealed hotspots and dimensions of variation in chemical structures, distinct overall structural groups, and parallels between molecules structural features and underlying biosynthesis. More widespread use of common reference frame molecular representation systems could hasten pathway elucidation efforts.

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BibTeXRIS

Babineau, N., Nguyen, L. T. D., Mathieu, D., McCue, C., Schlecht, N., Abrahamson, T., Hamberger, B., Busta, L.. 2024-10-01. A molecular representation system with a common reference frame for natural products pathway discovery and structural diversity tasks.. https://doi.org/10.1101/2024.10.01.616173

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