A repository of Ogden syndrome (NAA10) and NAA15 patient derived iPSC lines and isogenic pairs by X-chromosome screening and genome-editing.

Wesely, J.; Rusielewicz, T.; Chen, Y.-r.; Hartley, B.; McKenzie, D.; Yim, M.; Maguire, C. T.; Bia, R.; Franklin, S.; Makwana, R.; Marchi, E.; Nikte, M.; Patil, S.; Sapar, M.; Moroziewicz, D.; Global Stem Cell Array Team, N.; Lee, J.; Monsma, F.; Paull, D.; Lyon, G.

doi:10.1101/2024.09.28.615067

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bioRxiv · 10.1101/2024.09.28.615067

A repository of Ogden syndrome (NAA10) and NAA15 patient derived iPSC lines and isogenic pairs by X-chromosome screening and genome-editing.

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Abstract

Amino-terminal (Nt-) acetylation (NTA) is a common protein modification, affecting 80% of cytosolic proteins in humans. The human essential gene, NAA10, encodes the enzyme NAA10, as the catalytic subunit for the N-terminal acetyltransferase A (NatA) complex, including the accessory protein, NAA15. The first human disease directly involving NAA10 was discovered in 2011, and it was named Ogden syndrome (OS), after the location of the first affected family residing in Ogden, Utah, USA. Since that time, other variants have been found in NAA10 and NAA15. Here we describe the generation of 31 iPSC lines, with 16 from females and 15 from males. This cohort includes CRISPR-mediated correction to the wild-type genotype in 4 male lines, along with editing one female line to generate homozygous wild-type or mutant clones. Following the monoclonalizaiton and screening for X-chromosome activation status in female lines, 3 additional pairs of female lines, in which either the wild type allele is on the active X chromosome (Xa) or the pathogenic variant allele is on Xa, have been generated. Subsets of this cohort have been successfully used to make cardiomyocytes and neural progenitor cells (NPCs). These cell lines are made available to the community via the NYSCF Repository.

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Wesely, J., Rusielewicz, T., Chen, Y.-r., Hartley, B., McKenzie, D., Yim, M., Maguire, C. T., Bia, R., Franklin, S., Makwana, R., Marchi, E., Nikte, M., Patil, S., Sapar, M., Moroziewicz, D., Global Stem Cell Array Team, N., Lee, J., Monsma, F., Paull, D., Lyon, G.. 2024-09-28. A repository of Ogden syndrome (NAA10) and NAA15 patient derived iPSC lines and isogenic pairs by X-chromosome screening and genome-editing.. https://doi.org/10.1101/2024.09.28.615067

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