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bioRxiv · 10.1101/2024.09.26.615245

An amphiregulin reporter mouse enables transcriptional and clonal expansion analysis of reparative lung Treg cells

Loffredo, L. F.·Kaiser, K. A.·Kornberg, A.·Rao, S.·de los Santos-Alexis, K.·Han, A.·Arpaia, N.
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Abstract

Regulatory T (Treg) cells are known to play critical roles in tissue repair via provision of growth factors such as amphiregulin (Areg). Areg-producing Treg cells have previously been difficult to study because of an inability to isolate live Areg-producing cells. In this report, we created a novel reporter mouse to detect Areg expression in live cells (AregThy1.1). We employed influenza A and bleomycin models of lung damage to sort Areg-producing and -non-producing Treg cells for transcriptomic analyses. Single cell RNA-seq revealed distinct subpopulations of Treg cells and allowed transcriptomic comparisons of damage-induced populations. Single cell TCR sequencing showed that Treg cell clonal expansion is biased towards Areg-producing Treg cells, and largely occurs within damage-induced subgroups. Gene module analysis revealed functional divergence of Treg cells into immunosuppression-oriented and tissue repair-oriented groups, leading to identification of candidate receptors for induction of repair activity in Treg cells. We tested these using an ex vivo assay for Treg cell-mediated tissue repair, identifying 4-1BB agonism as a novel mechanism for reparative activity induction. Overall, we demonstrate that the AregThy1.1mouse is a promising tool for investigating tissue repair activity in leukocytes.

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Loffredo, L. F., Kaiser, K. A., Kornberg, A., Rao, S., de los Santos-Alexis, K., Han, A., Arpaia, N.. 2024-09-28. An amphiregulin reporter mouse enables transcriptional and clonal expansion analysis of reparative lung Treg cells. https://doi.org/10.1101/2024.09.26.615245

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