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bioRxiv · 10.1101/2024.09.20.614093

Unravelling the Proteomic Landscape of Imipenem Resistance in Pseudomonas aeruginosa: A Comparative Investigation between Clinical and Control Strains

Eidsaa, M.·Sharma, A.·Janasch, M.·Kuch, A.·Skoczynska, A.·di Bartolomeo, F.
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Abstract

The increasing prevalence of antimicrobial resistance (AMR) poses a significant challenge to global health, particularly with bacterial pathogens such as Pseudomonas aeruginosa, a notorious cause of nosocomial infections. This study focuses on the comparative proteomic analysis of an imipenem-resistant strain of P. aeruginosa, a representative of world epidemic clone ST235, and a wildtype control strain, P. aeruginosa ATCC 27853, in response to varying concentrations of imipenem. Using label-free quantification (LFQ) and gene ontology (GO) enrichment analyses, we identified significant differences in the proteomic responses between the two strains (data available via ProteomeXchange with identifier PXD055744.). The clinical strain exhibited a stable proteomic profile across the imipenem gradient, suggesting pre-established and efficient resistance mechanisms that do not require extensive reconfiguration under antibiotic pressure. In contrast, the control strain showed a broader, more reactive proteomic response, particularly in proteins associated with membrane transport, stress response, and biofilm formation. Notably, uncharacterized proteins were significantly upregulated in the clinical strain, indicating potential novel resistance mechanisms. These findings highlight the distinct strategies employed by the two strains, with the clinical strains stable resistance mechanisms contrasting sharply with the control strains reactive approach. The study underscores the importance of further research into the uncharacterized proteins that may play crucial roles in antibiotic resistance, potentially leading to new therapeutic targets in the fight against AMR. HighlightsO_LIStable resistance mechanisms: The clinical strain of Pseudomonas aeruginosa (ST235) exhibited a stable proteomic profile across varying concentrations of imipenem, indicating pre-established and efficient resistance mechanisms. C_LIO_LIReactive proteomic response: The control strain (P. aeruginosa ATCC 27853) showed a broader and more reactive proteomic response, particularly in proteins related to membrane transport, stress response, and biofilm formation. C_LIO_LIUncharacterized proteins: Significant upregulation of uncharacterized proteins in the clinical strain suggests potential novel resistance mechanisms that warrant further investigation. C_LI

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Eidsaa, M., Sharma, A., Janasch, M., Kuch, A., Skoczynska, A., di Bartolomeo, F.. 2024-09-20. Unravelling the Proteomic Landscape of Imipenem Resistance in Pseudomonas aeruginosa: A Comparative Investigation between Clinical and Control Strains. https://doi.org/10.1101/2024.09.20.614093

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