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bioRxiv · 10.1101/2024.09.12.612746

The Precise Basecalling of Short-Read Nanopore Sequencing

Wang, Z.·Tu, M.-J.·Song, C.·Liu, Z.·Wang, K. K.·Chen, S.·Yu, A.-M.·DING, H.
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Abstract

The nanopore sequencing of short sequences, whose lengths are typically less than 0.3kb therefore comparable with Illumina sequencing techniques, has recently gained wide attention. Here, we design a scheme for training nanopore basecallers that are specialized for short biomolecules. With bioengineered RNA (BioRNA) molecules as examples, we demonstrate the superior accuracy of basecallers trained by our scheme.

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Wang, Z., Tu, M.-J., Song, C., Liu, Z., Wang, K. K., Chen, S., Yu, A.-M., DING, H.. 2024-09-17. The Precise Basecalling of Short-Read Nanopore Sequencing. https://doi.org/10.1101/2024.09.12.612746

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