Vibrotactile auricular vagus nerve stimulation alters limbic system connectivity in humans: A pilot study

Donovan, K. M.; Adams, J. D.; Park, K. Y.; Demarest, P.; Tan, G.; Willie, J. T.; Brunner, P.; Gorlewicz, J. L.; Leuthardt, E. C.

doi:10.1101/2024.09.10.612366

bioRxiv · 10.1101/2024.09.10.612366

Vibrotactile auricular vagus nerve stimulation alters limbic system connectivity in humans: A pilot study

Donovan, K. M.·Adams, J. D.·Park, K. Y.·Demarest, P.·Tan, G.·Willie, J. T.·Brunner, P.·Gorlewicz, J. L.·Leuthardt, E. C.

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Abstract

Vibration offers a potential alternative modality for transcutaneous auricular vagus nerve stimulation (taVNS). However, mechanisms of action are not well-defined. The goal of this study was to evaluate the potential of vibrotactile stimulation as a method for activating central brain regions akin to other vagal nerve stimulation methodologies. To do so, intracranial electrophysiological signals were recorded in human subjects to perform a parametric characterization of vibrotactile taVNS and investigate changes in coherence across key brain regions. We hypothesized that vibrotactile taVNS would increase coherence between limbic brain areas, similar to areas activated by classic electrical VNS approaches. Our specific regions of interest included the orbitofrontal cortex, anterior cingulate cortex, amygdala, hippocampus, and parahippocampal gyrus. Patients with intractable epilepsy undergoing stereotactic electroencephalography (sEEG) monitoring participated in the study. Vibrotactile taVNS was administered across five vibration frequencies following a randomized stimulation on/off pattern, and sEEG signals were recorded throughout. Spectral coherence in response to stimulation was defined across four canonical frequency bands, theta, alpha, beta, and broadband gamma. At the group level, vibrotactile taVNS resulted in significantly increased global low-frequency coherence. Anatomically, multiple limbic brain regions exhibited notably increased coherence during taVNS compared to baseline. The percentage of total electrode pairs demonstrating increased coherence was also quantified at the individual level. 20 Hz vibration resulted in the highest percentage of responder pairs across low-frequency coherence measures, but notable inter-subject variability was present. Overall, vibrotactile taVNS induced significant low-frequency coherence increases involving several limbic system structures. Further, parametric characterization revealed the presence of inter-subject variability in terms of identifying the optimal vibration frequency. These findings encourage continued research into vibrotactile stimulation as an alternative modality for noninvasive vagus nerve stimulation.

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Donovan, K. M., Adams, J. D., Park, K. Y., Demarest, P., Tan, G., Willie, J. T., Brunner, P., Gorlewicz, J. L., Leuthardt, E. C.. 2024-09-14. Vibrotactile auricular vagus nerve stimulation alters limbic system connectivity in humans: A pilot study. https://doi.org/10.1101/2024.09.10.612366

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