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bioRxiv · 10.1101/2024.09.08.611941

Mechanisms of urate transport and uricosuric drugs inhibition in human URAT1

Guo, W.·Wei, M.·Li, Y.·Xu, J.·Zang, J.·Chen, Y.·Chen, L.
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Abstract

High urate levels in circulation lead to the accumulation of urate crystals in joints and ultimately inflammation and gout1. The reabsorption process of urate in the kidney by the urate transporter URAT1 plays a pivotal role in controlling serum urate levels2. Pharmacological inhibition of URAT1 by uricosuric drugs is a valid strategy for gout management3. Despite the clinical significance of URAT1, its structure and mechanism remain elusive. Here, we report the structures of human URAT1 (hURAT1) in complex with substrate urate or inhibitors benzbromarone and verinurad at resolution ranges from 3.0 to 3.3 [A]. Urate-bound hURAT1 adopts the outward-facing conformation. Urate is wrapped in the center of hURAT1 by five phenylalanines and coordinated by two positively charged residues on each side. Uricosuric compounds benzbromarone and verinurad occupy the urate-binding site of hURAT1 in the inward-facing conformation. Structural comparison between different conformations of hURAT1 reveals the rocker-switch-like mechanism for urate transport. Benzbromarone and verinurad exert their inhibitory effect by blocking not only the binding of urate but also the structural isomerization of hURAT1.

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Guo, W., Wei, M., Li, Y., Xu, J., Zang, J., Chen, Y., Chen, L.. 2024-09-09. Mechanisms of urate transport and uricosuric drugs inhibition in human URAT1. https://doi.org/10.1101/2024.09.08.611941

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