Nanomotion technology for testing azithromycin susceptibility of Salmonella enterica

Hinnu, M.; Mets, T.; Kerkez, I.; Putrins, M.; Kaldalu, N.; Cathomen, G.; Pla Verge, M.; Cichocka, D.; Sturm, A.; Tenson, T.; ERADIAMR,

doi:10.1101/2024.09.05.611511

bioRxiv · 10.1101/2024.09.05.611511

Nanomotion technology for testing azithromycin susceptibility of Salmonella enterica

Hinnu, M.·Mets, T.·Kerkez, I.·Putrins, M.·Kaldalu, N.·Cathomen, G.·Pla Verge, M.·Cichocka, D.·Sturm, A.·Tenson, T.·ERADIAMR,

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Abstract

Azithromycin is used to treat invasive salmonellosis, despite conflicting effective concentrations in vitro and in vivo. Resistance of Salmonella enterica to azithromycin is increasing. We demonstrate that nanomotion technology can be used for rapid phenotypic testing of Salmonellas susceptibility to azithromycin. Nanomotion changes under various culture conditions correlated with susceptibility measured by MIC determination, CFU counting, and fluorescent reporter-based estimates of intrabacterial azithromycin accumulation.

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Hinnu, M., Mets, T., Kerkez, I., Putrins, M., Kaldalu, N., Cathomen, G., Pla Verge, M., Cichocka, D., Sturm, A., Tenson, T., ERADIAMR,. 2024-09-06. Nanomotion technology for testing azithromycin susceptibility of Salmonella enterica. https://doi.org/10.1101/2024.09.05.611511

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