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bioRxiv · 10.1101/2024.08.30.610505

Developing Biosensors for Specific Assessment of Trans-translation in Pseudomonas aeruginosa.

L'Hermitte, B.·Chauvet, T.·Georgeault Daguenet, S.·Le Yondre, N.·Jehan, P.·Gillet, R.·Baysse, C.
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Abstract

Trans-translation is a crucial bacterial process and a target for new antibiotics. We developed two Pseudomonas aeruginosa biosensor strains that detect trans-translation inhibitors by exploiting the bacteriums natural red fluorescence, linked to protoporphyrin IX accumulation. The first biosensor monitors tmRNA-SmpB-mediated tagging, while the second serves as control for biosensor 1 by keeping track of ClpP1-related proteolysis and porphyrin biosynthesis. Validation through gene deletions and complementation confirmed their specificity. These biosensors were effective in screening antibiotics and designed inhibitors, demonstrating their potential for high-throughput identification of trans-translation inhibitors in drug-resistant P. aeruginosa.

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L'Hermitte, B., Chauvet, T., Georgeault Daguenet, S., Le Yondre, N., Jehan, P., Gillet, R., Baysse, C.. 2024-08-31. Developing Biosensors for Specific Assessment of Trans-translation in Pseudomonas aeruginosa.. https://doi.org/10.1101/2024.08.30.610505

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