A wheat tandem kinase and NLR pair confers resistance to multiple fungal pathogens

Lu, P.; Zhang, G.; Li, J.; Gong, Z.; Wang, G.; Dong, L.; Zhang, H.; Guo, G.; Su, M.; Wang, Y.; Zhu, K.; Wu, Q.; Chen, Y.; Li, M.; Huang, B.; Li, B.; Li, W.; Hou, Y.; Cui, X.; Fu, H.; Qiu, D.; Yuan, C.; Li, H.; Zhou, J.; Han, G. z.; Chen, Y.-h.; Liu, Z.

doi:10.1101/2024.08.30.610185

bioRxiv · 10.1101/2024.08.30.610185

A wheat tandem kinase and NLR pair confers resistance to multiple fungal pathogens

Lu, P.·Zhang, G.·Li, J.·Gong, Z.·Wang, G.·Dong, L.·Zhang, H.·Guo, G.·Su, M.·Wang, Y.·Zhu, K.·Wu, Q.·Chen, Y.·Li, M.·Huang, B.·Li, B.·Li, W.·Hou, Y.·Cui, X.·Fu, H.·Qiu, D.·Yuan, C.·Li, H.·Zhou, J.·Han, G. z.·Chen, Y.-h.·Liu, Z.

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Abstract

Recently discovered tandem kinase proteins (TKPs) are pivotal to the innate immune systems of cereal plants, yet how they initiate plant immune responses remains unclear. This report identifies the wheat protein WTN1, a non-canonical NLR receptor featuring tandem NB-ARC domains, as crucial for WTK3-mediated disease resistance. Both WTK3 and its allelic variant Rwt4, known for conferring resistance to wheat powdery mildew and blast respectively, are capable of recognizing the blast effector PWT4, and activate WTN1 to form calcium-permeable channels, akin to ZAR1 and Sr35. This study unveils a unique plant defense mechanism wherein TKPs and associated NLRs operate as "sensor-executor" pairs against fungal pathogens. Additionally, evolutionary analyses reveal a co-evolutionary trajectory of the TKP-NLR module, highlighting their synergistic role in triggering plant immunity. One Sentence SummaryAn ancient synergistic TKP-NLR pair triggers innate immunity for multiple disease resistance in wheat.

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Lu, P., Zhang, G., Li, J., Gong, Z., Wang, G., Dong, L., Zhang, H., Guo, G., Su, M., Wang, Y., Zhu, K., Wu, Q., Chen, Y., Li, M., Huang, B., Li, B., Li, W., Hou, Y., Cui, X., Fu, H., Qiu, D., Yuan, C., Li, H., Zhou, J., Han, G. z., Chen, Y.-h., Liu, Z.. 2024-08-30. A wheat tandem kinase and NLR pair confers resistance to multiple fungal pathogens. https://doi.org/10.1101/2024.08.30.610185

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