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bioRxiv · 10.1101/2024.08.28.609956

Fast Low-Input Efficient Hi-C

Guetta, M.·Avidan, N.·Kariti, H.·Davis, L.·Shemer, R.·Elliot, E.·Kaplan, N.
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Abstract

We developed FLIE (Fast Low-Input Efficient) Hi-C, a highly optimized and simplified version of Hi-C. Our method shortens the duration of Hi-C from five days to two days and decreases key reagent amounts 5-20 fold, while maintaining experimental controls and data quality. Importantly, the method also reduces the required input material by 100-1000 fold, as we demonstrate by generating detailed high complexity interaction maps from low-input sorted mouse frozen neuron nuclei and from 5,000-10,000 human Hap1 cells.

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Guetta, M., Avidan, N., Kariti, H., Davis, L., Shemer, R., Elliot, E., Kaplan, N.. 2024-08-29. Fast Low-Input Efficient Hi-C. https://doi.org/10.1101/2024.08.28.609956

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