Conjugation of dn-OPDA with amino acids inhibits its hormonal bioactivity in Marchantia polymorpha

Liang, W.; Zamarreno, A. M.; Torres-Montilla, S.; de la Torre, A.; Totozafy, J. C.; Kaji, T.; Ueda, M.; Corso, M.; Garcia-Mina, J. M.; Solano, R.; Chini, A.

doi:10.1101/2024.08.27.609933

bioRxiv · 10.1101/2024.08.27.609933

Conjugation of dn-OPDA with amino acids inhibits its hormonal bioactivity in Marchantia polymorpha

Liang, W.·Zamarreno, A. M.·Torres-Montilla, S.·de la Torre, A.·Totozafy, J. C.·Kaji, T.·Ueda, M.·Corso, M.·Garcia-Mina, J. M.·Solano, R.·Chini, A.

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Abstract

Jasmonates are important phytohormones activating plant tolerance to biotic and abiotic stress, as well as different development processes. A conserved signalling pathway activated by distinct hormones in different plant species mediates these responses: dinor-12-oxo-phytodienoic acid (dn-OPDA) isomers in bryophytes and lycophytes, and JA-Ile in most vascular plants. The final responses depend, in many cases, on the accumulation of specialized metabolites. To identify novel compounds regulated by the dn-OPDA pathway in Marchantia, untargeted metabolomic analyses were carried out in response to dn-OPDA-regulated stress. A novel group of molecules were identified as dn-OPDA-amino acid conjugates (dn-OPDA-aas), and their accumulation after wounding and herbivory confirmed by targeted metabolic profiling in Marchantia and all species in which we previously found dn-iso-OPDA. Mutants in GRETCHEN-HAGEN 3A (MpGH3A) failed to accumulate dn-OPDA-aa conjugates, and showed a constitutive activation of the OPDA pathway and increased resistance to herbivory. Our results show that dn-iso-OPDA bioactivity is reduced by conjugation with amino acids. Therefore, a dichotomous role of jasmonate conjugation in land plants is highlighted: jasmonic acid (JA) conjugation with isoleucine (Ile) produce the bioactive JA-Ile in tracheophytes, whereas conjugation of dn-iso-OPDA with different amino acids disactivate the hormone in bryophytes and lycophytes.

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Liang, W., Zamarreno, A. M., Torres-Montilla, S., de la Torre, A., Totozafy, J. C., Kaji, T., Ueda, M., Corso, M., Garcia-Mina, J. M., Solano, R., Chini, A.. 2024-08-30. Conjugation of dn-OPDA with amino acids inhibits its hormonal bioactivity in Marchantia polymorpha. https://doi.org/10.1101/2024.08.27.609933

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