An alternative mechanism for activation of innate immune signaling by MDA5

Quack, S.; Maity, S.; America, P. P. B.; Klein, M.; Herrero del Valle, A.; Singh, R.; Smitskamp, Q.; Papini, F. S.; Broedersz, C.; Roos, W.; Modis, Y.; Dulin, D.

doi:10.1101/2024.08.27.609867

bioRxiv · 10.1101/2024.08.27.609867

An alternative mechanism for activation of innate immune signaling by MDA5

Quack, S.·Maity, S.·America, P. P. B.·Klein, M.·Herrero del Valle, A.·Singh, R.·Smitskamp, Q.·Papini, F. S.·Broedersz, C.·Roos, W.·Modis, Y.·Dulin, D.

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Abstract

Long double-stranded (ds) RNA in the cytosol acts as a potent inflammatory molecule recognized by the receptor MDA5, triggering the innate immune response. Mutations affecting MDA5 ATPase activity lead to severe pathological conditions. MDA5 nucleoprotein filament assembly-disassembly dynamic is proposed to regulate dsRNA recognition, though the exact mechanism remains unclear. Here, we employed magnetic tweezers to monitor the assembly and manipulate MDA5 filaments at the single dsRNA level. Following a slow nucleation event, MDA5 assembles cooperatively and directionally into (partial) filaments and utilizes ATP hydrolysis to compact dsRNA via single-stranded (ss) RNA loop extrusion, even against a significant opposing force. This compacted state is further stabilized by oligomerization of MDA5s caspase recruitment domain (CARD) and requires high force to be disrupted. ssRNA gaps impaired compaction, suggesting a new mechanism for dsRNA recognition. We propose that MDA5 mediated dsRNA compaction captures viral dsRNA, preventing further usage for viral replication.

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Quack, S., Maity, S., America, P. P. B., Klein, M., Herrero del Valle, A., Singh, R., Smitskamp, Q., Papini, F. S., Broedersz, C., Roos, W., Modis, Y., Dulin, D.. 2024-08-27. An alternative mechanism for activation of innate immune signaling by MDA5. https://doi.org/10.1101/2024.08.27.609867

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