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bioRxiv · 10.1101/2024.08.23.609327

Monitoring DNA double strand break repair in Saccharomyces cerevisiae at high throughput and high resolution

Shalev Ezra, Y.·Saguy, A.·Levin, G.·Weiss, L.·Alalouf, O.·Shechtman, Y.
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Abstract

DNA repair is critical for cellular function and genomic stability across organisms. Yeast mating-type switching serves as an established model for studying DNA break repair and chromatin dynamics. However, real-time tracking of mating-type switching in live cells remains challenging due to resolution limitations of existing techniques. Here, we use high-throughput methods, including three-dimensional imaging, to follow the dynamics of DNA damage and repair and to quantify mating-type switching occurrences at the single live cell level, with unprecedented resolution. We reveal chromatin reconfiguration for both single- and double-strand breaks following switching induction. Our findings provide new observation of the correlation between chromatin folding and single-strand breaks.

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Shalev Ezra, Y., Saguy, A., Levin, G., Weiss, L., Alalouf, O., Shechtman, Y.. 2024-08-23. Monitoring DNA double strand break repair in Saccharomyces cerevisiae at high throughput and high resolution. https://doi.org/10.1101/2024.08.23.609327

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