Primary culture of high grade serous ovarian cancer cells, selection and derivation of three cell lines

Brenton, J. D.; Vias, M.; Sauer, C. M.; Funingana, G.; Piskorz, A. M.; Moore, E.; Chilamakuri, C.; Hall, D.; Goranova, T.; Van Oudenhove, E.; Earl, H. M.; Goldlust, I.

doi:10.1101/2024.08.15.607946

bioRxiv · 10.1101/2024.08.15.607946

Primary culture of high grade serous ovarian cancer cells, selection and derivation of three cell lines

Brenton, J. D.·Vias, M.·Sauer, C. M.·Funingana, G.·Piskorz, A. M.·Moore, E.·Chilamakuri, C.·Hall, D.·Goranova, T.·Van Oudenhove, E.·Earl, H. M.·Goldlust, I.

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Abstract

Cell line models for high grade serous ovarian cancer (HGSOC) are limited in number and are poorly clinically annotated. Consequently, existing models often fail to recapitulate common features of HGSOC, inhibiting mechanistic and therapeutic discovery. We generated and characterised three spontaneously immortalized continuous HGSOC cell lines named CIOV1, CIOV2, and CIOV3 and confirmed that each cell line retained the genomic and pathologic characteristics of its parental tumour. We show that subclonal cell populations present at initiation, expanded and contracted during the culturing process before converging on a stable immortalized line. These lines are new valuable models to study acquired chemoresistance of HGSOC.

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Brenton, J. D., Vias, M., Sauer, C. M., Funingana, G., Piskorz, A. M., Moore, E., Chilamakuri, C., Hall, D., Goranova, T., Van Oudenhove, E., Earl, H. M., Goldlust, I.. 2024-08-19. Primary culture of high grade serous ovarian cancer cells, selection and derivation of three cell lines. https://doi.org/10.1101/2024.08.15.607946

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