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bioRxiv · 10.1101/2024.08.14.607933

Immune checkpoint inhibitor treatment does not impair ovarian or endocrine function in a mouse model of triple negative breast cancer

De La Cruz, P.·Woodman-Sousa, M. F.·McAdams, J. N.·Sweeney, E.·Hakim, L.·Morales Aquino, M.·Grive, K. J.
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Abstract

BackgroundRepresenting 15-20% of all breast cancer cases, triple negative breast cancer (TNBC) is diagnosed more frequently in reproductive-age women and exhibits higher rates of disease metastasis and recurrence when compared with other subtypes. Few targeted treatments exist for TNBC, and many patients experience infertility and endocrine disruption as a result of frontline chemotherapy treatment. While they are a promising option for less toxic therapeutic approaches, little is known about the effects of immune checkpoint inhibitors on reproductive and endocrine function. ResultsOur findings in a syngeneic TNBC mouse model revealed that therapeutically relevant immunotherapies targeting PD-1, LAG-3, and TIM-3 had no effect on the quality and abundance of ovarian follicles, estrus cyclicity, or hormonal homeostasis. Similarly, in a tumor-free mouse model, we found that ovarian architecture, follicle abundance, estrus cyclicity, and ovulatory efficiency remain unchanged by PD-1 blockade. ConclusionsTaken together, our results suggest that immunotherapy may be a promising component of fertility-sparing therapeutic regimens for patients that wish to retain ovarian and endocrine function after cancer treatment.

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De La Cruz, P., Woodman-Sousa, M. F., McAdams, J. N., Sweeney, E., Hakim, L., Morales Aquino, M., Grive, K. J.. 2024-08-19. Immune checkpoint inhibitor treatment does not impair ovarian or endocrine function in a mouse model of triple negative breast cancer. https://doi.org/10.1101/2024.08.14.607933

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