Porcine Nasal Organoids as a model to study the interactions between the swine nasal microbiota and the host

Bonillo-Lopez, L.; Carmona-Vicente, N.; Tarres-Freixas, F.; Kochanowski, K.; Martinez, J.; Perez, M.; Sibila, M.; Correa-Fiz, F.; Aragon, V.

doi:10.1101/2024.08.13.606910

bioRxiv · 10.1101/2024.08.13.606910

Porcine Nasal Organoids as a model to study the interactions between the swine nasal microbiota and the host

Bonillo-Lopez, L.·Carmona-Vicente, N.·Tarres-Freixas, F.·Kochanowski, K.·Martinez, J.·Perez, M.·Sibila, M.·Correa-Fiz, F.·Aragon, V.

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Abstract

Interactions between the nasal epithelium, commensal nasal microbiota, and respiratory pathogens play a key role in respiratory infections. Currently there is a lack of experimental models to study such interactions under defined in vitro conditions. Here, we developed a Porcine Nasal Organoid (PNO) system from nasal tissue of pigs as well as from cytological brushes. PNOs exhibited similar structure and cell types than the nasal mucosa, as evaluated by immunostaining. PNOs were inoculated with porcine commensal strains of Moraxella pluranimalium, Rothia nasimurium and the pathobiont Glaesserella parasuis for examining host-commensal-pathogen interactions. All strains adhered to the PNOs, although at different levels. M. pluranimalium and G. parasuis strains stimulated the production of proinflammatory cytokines, whereas R. nasimurium induced the production of IFN{gamma} and diminished the proinflammatory effect of the other strains. Overall, PNOs mimic the in vivo nasal mucosa and can be useful to perform host-microbe interaction studies. In briefInteractions between nasal epithelium, commensal nasal microbiota, and respiratory pathogens play a key role in respiratory infections. We developed porcine nasal organoids to mimic the nasal mucosa and use as a model to study those interactions. Additionally, this development supports the reduction of the number of animals for animal experimentation. HighlightsO_LIFirst generation of Porcine Nasal Organoids (PNOs) from nasal turbinates and swabs C_LIO_LIPNOs recapitulate features of in vivo tissue and maintain self-renewal capacity C_LIO_LIHost-microbe interactions can be studied using the PNO system C_LIO_LIRothia nasimurium inhibits the inflammation induced by other bacteria C_LI

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Bonillo-Lopez, L., Carmona-Vicente, N., Tarres-Freixas, F., Kochanowski, K., Martinez, J., Perez, M., Sibila, M., Correa-Fiz, F., Aragon, V.. 2024-08-13. Porcine Nasal Organoids as a model to study the interactions between the swine nasal microbiota and the host. https://doi.org/10.1101/2024.08.13.606910

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