Engineered Nanotopographies Induce Transient Openings in the Nuclear Membrane

Sarikhani, E.; Patel, V.; Li, Z.; Meganathan, D. P.; Rahmani, K.; Sadr, L.; Hosseini, R.; Visda, D.; Shukla, S.; Naghsh-Nilchi, H.; Balaji, A.; McMahon, G.; Chen, S.; Schoneberg, J.; McHugh, C. A.; Shi, L.; Jahed, Z.

doi:10.1101/2024.08.13.605467

bioRxiv · 10.1101/2024.08.13.605467

Engineered Nanotopographies Induce Transient Openings in the Nuclear Membrane

Sarikhani, E.·Patel, V.·Li, Z.·Meganathan, D. P.·Rahmani, K.·Sadr, L.·Hosseini, R.·Visda, D.·Shukla, S.·Naghsh-Nilchi, H.·Balaji, A.·McMahon, G.·Chen, S.·Schoneberg, J.·McHugh, C. A.·Shi, L.·Jahed, Z.

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Abstract

Materials with engineered nano-scale surface topographies, such as nanopillars, nanoneedles, and nanowires, mimic natural structures like viral spike proteins, enabling them to bypass biological barriers like the plasma membrane. These properties have led to applications in nanoelectronics for intracellular sensing and drug delivery platforms, some of which are already in clinical trials. Here, we present evidence that nanotopographic materials can induce transient openings in the nuclear membranes of various cell types without penetrating the cells, breaching the nucleo-cytoplasmic barrier and allowing uncontrolled molecular exchange across the nuclear membrane. These openings, induced by nanoscale curvature, are temporary and repaired through ESCRT-mediated mechanisms. Our findings suggest a potential for nano topographic materials for direct nuclear sensing and delivery, holding promise for improving the delivery, efficiency, and safety of therapeutic agents to the nucleus.

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Sarikhani, E., Patel, V., Li, Z., Meganathan, D. P., Rahmani, K., Sadr, L., Hosseini, R., Visda, D., Shukla, S., Naghsh-Nilchi, H., Balaji, A., McMahon, G., Chen, S., Schoneberg, J., McHugh, C. A., Shi, L., Jahed, Z.. 2024-08-16. Engineered Nanotopographies Induce Transient Openings in the Nuclear Membrane. https://doi.org/10.1101/2024.08.13.605467

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