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bioRxiv · 10.1101/2024.08.11.607476

Selective regulation of IFN-γ and IL-4 co-producing unconventional T cells by purinergic signalling

Abstract

Unconventional T cells, including mucosal-associated invariant T (MAIT), natural killer T (NKT), and gamma-delta T ({gamma}{delta}T) cells, comprise distinct T-bet+, IFN-{gamma}+ and ROR{gamma}t+, IL-17+ subsets which play differential roles in health and disease. NKT1 cells are susceptible to ARTC2-mediated P2X7 receptor (P2RX7) activation, but the effects on other unconventional T-cell types are unknown. Here, we show that MAIT, {gamma}{delta}T, and NKT cells express P2RX7 and are sensitive to P2RX7-mediated cell death. Mouse peripheral T-bet+ MAIT1, {gamma}{delta}T1, and NKT1 cells, especially in liver, co-express ARTC2 and P2RX7, which can be further upregulated by retinoic acid. Blocking ARTC2 or inhibiting P2RX7 protected MAIT1, {gamma}{delta}T1, and NKT1 cells from cell death, enhanced their survival in vivo, and increased the number of IFN-{gamma}-secreting cells without affecting IL-17 production. Importantly, this revealed the existence of IFN-{gamma} and IL-4 co-producing unconventional T-cell populations normally lost upon isolation due to ARTC2/P2RX7-induced death. Administering extracellular NAD in vivo activated this pathway, depleting P2RX7-sensitive unconventional T cells. Our study reveals ARTC2/P2RX7 as a common regulatory axis modulating the unconventional T-cell compartment, affecting the viability of IFN-{gamma}- and IL-4-producing T cells, offering important insights to facilitate future studies into how these cells can be regulated in health and disease.

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BibTeXRIS

Xu, C., Obers, A., Qin, M., Brandli, A., Wong, J., Huang, X., Clatch, A., Fayed, A., Starkey, G., DCosta, R., Gordon, C. L., Beattie, L., Mackay, L. K., Godfrey, D. I., Koay, H.-F.. 2024-08-11. Selective regulation of IFN-γ and IL-4 co-producing unconventional T cells by purinergic signalling. https://doi.org/10.1101/2024.08.11.607476

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