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bioRxiv · 10.1101/2024.07.30.605819

Muscarinic receptor activation preferentially inhibits rebound in vulnerable dopaminergic neurons

Beaver, M. L.·Evans, R. C.
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Abstract

Dopaminergic subpopulations of the substantia nigra pars compacta (SNc) differentially degenerate in Parkinsons disease and are characterized by unique electrophysiological properties. The vulnerable population expresses a T-type calcium channel-mediated afterdepolarization (ADP) and shows rebound activity upon release from inhibition, whereas the resilient population does not have an ADP and is slower to fire after hyperpolarization. This rebound activity can trigger dopamine release in the striatum, an important component of basal ganglia function. Using whole-cell patch clamp electrophysiology on ex vivo slices from adult mice of both sexes, we find that muscarinic activation with the non-selective muscarinic agonist Oxotremorine inhibits rebound activity more strongly in vulnerable vs resilient SNc neurons. Here, we show that this effect depends on the direct activation of muscarinic receptors on the SNc dopaminergic neurons. Through a series of pharmacological and transgenic knock-out experiments, we tested whether the muscarinic inhibition of rebound was mediated through the canonical rebound-related ion channels: T-type calcium channels, hyperpolarization-activated cation channels (HCN), and A-type potassium channels. We find that muscarinic receptor activation inhibits HCN-mediated current (Ih) in vulnerable SNc neurons, but that Ih activity is not necessary for the muscarinic inhibition of rebound activity. Similarly, we find that Oxotremorine inhibits rebound activity independently of T-type calcium channels and A-type potassium channels. Together these findings reveal new principles governing acetylcholine and dopamine interactions, showing that muscarinic receptors directly affect SNc rebound activity in the midbrain at the somatodendritic level and differentially modify information processing in distinct SNc subpopulations. Significance StatementDopaminergic neurons in the substantia nigra pars compacta (SNc) can be divided into functional subpopulations with distinct basal ganglia connectivity and different degeneration patterns in Parkinsons disease. We show that the vulnerable and resilient subpopulations of SNc dopaminergic neurons are differentially modulated by muscarinic receptor activation. Specifically, muscarinic receptor activation inhibits rebound activity more strongly in the vulnerable SNc neurons than in the resilient. We find that this inhibition occurs through a non-canonical rebound-related pathway and is not mediated through the channels best known for modulating rebound in midbrain dopaminergic neurons. These findings are important because they reveal novel acetylcholine-dopamine interactions that occur in the midbrain and affect information processing in distinct basal ganglia circuits.

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Beaver, M. L., Evans, R. C.. 2024-07-31. Muscarinic receptor activation preferentially inhibits rebound in vulnerable dopaminergic neurons. https://doi.org/10.1101/2024.07.30.605819

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