Effect of curcumin and 5-bromoindole-3-carboxyaldehyde on the inhibition of Quorum sensig-dependent virulence factors in Pseudomonas aeruginosa

Gonzalez, G. M.; Garcia-Contreras, R.; Garcia Vales, I.; Valdes, N. G.; Lugo Gutierrez, D. A.; Bustamante Martinez, I. L.; Almeida, J.

doi:10.1101/2024.07.29.605599

bioRxiv · 10.1101/2024.07.29.605599

Effect of curcumin and 5-bromoindole-3-carboxyaldehyde on the inhibition of Quorum sensig-dependent virulence factors in Pseudomonas aeruginosa

Gonzalez, G. M.·Garcia-Contreras, R.·Garcia Vales, I.·Valdes, N. G.·Lugo Gutierrez, D. A.·Bustamante Martinez, I. L.·Almeida, J.

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Abstract

Curcumin and 5-bromoindole-3-carboxaldehyde were tested for their ability to inhibit quorum sensing-dependent virulence factors in Pseudomonas aeruginosa strains Pa01, Pa14, and ATCC 27853. The compounds were evaluated at curcumin (1, 5 g/ml) and 5-bromoindole-3-carboxaldehyde (30, 50, 100 g/ml). Virulence factors--exoprotease, elastase, pyocyanin, and alginate --were measured. Chemoinformatics properties were analyzed using Molinspiration, Molsoft, Osiris Property Explorer, pkCSM, and SwissADME. Results showed 5-bromoindole-3-carboxaldehyde significantly reduced pyocyanin in Pa01 (92.5% at 100 g/ml), elastase (79.85% at 100 g/ml), exoprotease (92.8% at 50 g/ml), and alginate in ATCC 27853 (76.16% at 100 g/ml). Curcumin effectively inhibited pyocyanin in ATCC 27853 (100% at 5 g/ml), elastase in Pa14 (56.19% at 5 g/ml), exoprotease in Pa01 (87.04% at 5 g/ml), and alginate (48.26% at 5 g/ml). Both compounds demonstrated strong inhibition of virulence factors, suggesting their potential as anti-virulence agents against P. aeruginosa, a highly resistant bacterium prevalent in nosocomial infections.

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Gonzalez, G. M., Garcia-Contreras, R., Garcia Vales, I., Valdes, N. G., Lugo Gutierrez, D. A., Bustamante Martinez, I. L., Almeida, J.. 2024-07-29. Effect of curcumin and 5-bromoindole-3-carboxyaldehyde on the inhibition of Quorum sensig-dependent virulence factors in Pseudomonas aeruginosa. https://doi.org/10.1101/2024.07.29.605599

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