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bioRxiv · 10.1101/2024.07.26.605276

A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency

Miyatake, Y.·Ikawa, T.·Yanagiya, R.·Kotaki, R.·Kameda, K.·Nasu, R. K.·Okuyama, K.·Hirano, K.-i.·Hosokawa, H.·Hozumi, K.·Ohtsuka, M.·Kisikawa, T.·Shibata, C.·Otsuka, M.·Maruyama, R.·Ando, K.·Kurosaki, T.·Kawamoto, H.·Kotani, A.
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Abstract

Accumulated studies have reported that hematopoietic differentiation was primarily regulated by transcription factors. Early B cell factor 1 (EBF1) is an essential transcription factor for B lymphopoiesis. Contrary to the canonical notion, we found that a single miRNA, miRNA-195 (miR-195) transduction let EBF1 deficient hematopoietic progenitor cells (HPCs) express CD19, carry out V(D)J recombination and class switch recombination, which implied that B cell matured without EBF1. A part of the mechanism was caused by FOXO1 accumulation via inhibition of FOXO1 phosphorylation pathways in which targets of miR-195 are enriched. These results suggested that some miRNA transductions could function as alternatives to transcription factors.

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Miyatake, Y., Ikawa, T., Yanagiya, R., Kotaki, R., Kameda, K., Nasu, R. K., Okuyama, K., Hirano, K.-i., Hosokawa, H., Hozumi, K., Ohtsuka, M., Kisikawa, T., Shibata, C., Otsuka, M., Maruyama, R., Ando, K., Kurosaki, T., Kawamoto, H., Kotani, A.. 2024-07-26. A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency. https://doi.org/10.1101/2024.07.26.605276

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