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bioRxiv · 10.1101/2024.07.25.605163

Cell surface ribonucleoproteins cluster with heparan sulfate to regulate growth factor signaling

Abstract

Receptor-ligand interactions govern a wide array of biological pathways, facilitating a cells ability to interrogate and integrate information from the extracellular space. Here, using an unbiased genome-wide knockout screen, we identify heparan sulfate proteoglycans (HSPGs) as a major component in the organizational mechanism of cell surface glycoRNA and cell surface RNA binding proteins (csRBPs). Cleavage of mature heparan sulfate chains, knockout of N- and 6-O-sulfotransferases, overexpression of endo-6-O-sulfatases, or the addition of exogenous heparan sulfate chains with high 2-O sulfation result in marked loss in glycoRNA-csRBP clustering in U2OS cells. Functionally, we provide evidence that signal transduction by HS-dependent growth factors such as VEGF-A165 is regulated by cell surface RNAs, and in vitro VEGF-A165, selectively interacts with glycoRNAs. Our findings uncover a new molecular mechanism of controlling signal transduction of specific growth factors across the plasma membrane by the regulated assembly of glycoRNAs, csRBPs, and heparan sulfate clusters.

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Flynn, R. A., Chair, P., Perr, J., Kageler, L., Lebedenko, C. G., Dias, J. M. L., Yankova, E., Esko, J. D., Tzelepis, K.. 2024-07-25. Cell surface ribonucleoproteins cluster with heparan sulfate to regulate growth factor signaling. https://doi.org/10.1101/2024.07.25.605163

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