An orexin-sensitive subpopulation of layer 6 neurons regulates cortical excitability and anxiety behaviour.

Messore, F.; Therpurakal Narayanan, R.; Dufour, J. P.; Hoerder-suabedissen, A.; Guidi, L.; Korrell, K.; Mueller, M.; Lak, A.; Bannerman, D.; Mann, E. O.; Molnar, Z.

doi:10.1101/2024.07.25.605138

bioRxiv · 10.1101/2024.07.25.605138

An orexin-sensitive subpopulation of layer 6 neurons regulates cortical excitability and anxiety behaviour.

Messore, F.·Therpurakal Narayanan, R.·Dufour, J. P.·Hoerder-suabedissen, A.·Guidi, L.·Korrell, K.·Mueller, M.·Lak, A.·Bannerman, D.·Mann, E. O.·Molnar, Z.

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Abstract

Cortical layer 6 (L6) neurons uniquely respond to orexin - a neuropeptide influencing arousal and emotion. We show that Drd1a-Cre+ neurons in the prefrontal cortex are selectively sensitive to orexin and regulate prefrontal network activation in vitro and in vivo. Chronically silencing these neurons impairs orexin-induced prefrontal activation and reduces anxiety-like behaviour, indicating that orexin-responsive L6 neurons modulate emotional states and may be a substrate for anxiety regulation.

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Messore, F., Therpurakal Narayanan, R., Dufour, J. P., Hoerder-suabedissen, A., Guidi, L., Korrell, K., Mueller, M., Lak, A., Bannerman, D., Mann, E. O., Molnar, Z.. 2024-07-25. An orexin-sensitive subpopulation of layer 6 neurons regulates cortical excitability and anxiety behaviour.. https://doi.org/10.1101/2024.07.25.605138

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