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bioRxiv · 10.1101/2024.07.24.604735

Mapping the space of protein binding sites with sequence-based protein language models

Abstract

Binding sites are the key interfaces that determine a proteins biological activity, and therefore common targets for therapeutic intervention. Techniques that help us detect, compare and contextualise binding sites are hence of immense interest to drug discovery. Here we present an approach that integrates protein language models with a 3D tesselation technique to derive rich and versatile representations of binding sites that combine functional, structural and evolutionary information with unprecedented detail. We demonstrate that the associated similarity metrics induce meaningful pocket clusterings by balancing local structure against global sequence effects. The resulting embeddings are shown to simplify a variety of downstream tasks: they help organise the "pocketome" in a way that efficiently contextualises new binding sites, construct performant druggability models, and define challenging train-test splits for believable benchmarking of pocket-centric machine-learning models.

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BibTeXRIS

Oruc, T., Kadukova, M., Davies, T. G., Verdonk, M., Poelking, C.. 2024-07-24. Mapping the space of protein binding sites with sequence-based protein language models. https://doi.org/10.1101/2024.07.24.604735

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