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bioRxiv · 10.1101/2024.07.21.604148

SCAR-6 elncRNA locus epigenetically regulates PROZ and modulates coagulation and vascular function

Ranjan, G.·Sehgal, P.·Scaria, V.·Sivasubbu, S.
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Abstract

Syntenic conservation is an effective strategy to identify evolutionarily conserved lncRNA orthologs. In this study, we identified a novel uncharacterized conserved lncRNA known as Syntenic Cardiovascular Conserved Region-Associated lncRNA-6 (scar-6) and functionally validated its role in coagulation and cardiovascular function. Precise editing of the scar-6 lncRNA locus in zebrafish (scar-6gib007{Delta}12/{Delta}12) resulted in cranial hemorrhage and permeability defects. Further analysis, including overexpression, locus editing, and rescue experiments, provided compelling evidence for the critical role of the scar-6 transcript in the coagulation process of zebrafish. Notably, rescue attempts were unsuccessful in mitigating cranial hemorrhage. Molecular investigation revealed that the scar-6 RNA acts as an enhancer lncRNA (elncRNA), and controls the expression of prozb, an inhibitor of factor Xa, through the enhancer element on its locus. The scar-6 locus actively suppresses the loop formation between prozb and scar-6 sequences, facilitated by methylation of CpG island via the prdm14-PRC2 complex, which is stabilized by the scar-6 elncRNA transcript. Disruption of this mechanism in scar-6gib007{Delta}12/{Delta}12 zebrafish led to impaired vascular function and subsequent hemorrhage. This was triggered by the activation of the PAR2 receptor mediated by upregulation of prozb, which in turn caused NF-{kappa}B-mediated endothelial cell activation. This study presents novel evidence for the multifaceted function of the scar-6 locus, highlighting its crucial role in regulating the coagulation cascade gene prozb and maintaining homeostasis and vascular function. Synopsis O_FIG O_LINKSMALLFIG WIDTH=177 HEIGHT=200 SRC="FIGDIR/small/604148v1_ufig1.gif" ALT="Figure 1"> View larger version (38K): org.highwire.dtl.DTLVardef@ea55e4org.highwire.dtl.DTLVardef@ad01beorg.highwire.dtl.DTLVardef@4da1eborg.highwire.dtl.DTLVardef@c153a9_HPS_FORMAT_FIGEXP M_FIG C_FIG ProZ-PZI is a natural inhibitor of activated coagulation factor X (F10) and plays a major role in maintaining hemostasis in-vivo. Here, the novel evolutionary syntenic conserved scar-6 elncRNA locus is shown to regulate prozb expression and control coagulation and vascular integrity in zebrafish. O_LIThe scar-6 acts as an enhancer lncRNA (elncRNA). It controls prozb expression and modulates coagulation and vascular function in zebrafish. C_LIO_LIThe scar-6 elncRNA stabilizes the Prdm14-PRC2 complex binding to scar-6 locus. This inhibits prozb/scar-6 looping via methylating the CpG island under wildtype conditions. C_LIO_LIOverexpressed prozb in scar-6 edited animals activates PAR2 receptor, causing endothelial cell activation and vascular dysfunction. C_LI

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Ranjan, G., Sehgal, P., Scaria, V., Sivasubbu, S.. 2024-07-22. SCAR-6 elncRNA locus epigenetically regulates PROZ and modulates coagulation and vascular function. https://doi.org/10.1101/2024.07.21.604148

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