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bioRxiv · 10.1101/2024.07.20.604410

Lung structural cell dynamics are altered by influenza virus infection experience leading to rapid immune protection following viral re-challenge

Worrell, J. C.·Hargrave, K. E.·Finney, G. E.·Hansell, C.·Cole, J. J.·Singh Niijar, J.·Morton, F. R.·Pingen, M.·Purnell, T.·Mitchelson, K.·Brennan, E.·Allan, J.·Ilia, G.·Herder, V.·Kennedy Dietrich, C.·Doncheva, Y.·Jamieson, N.·MacLeod, M. K.
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Abstract

Lung structural cells, including epithelial cells and fibroblasts, form barriers against pathogens and trigger immune responses following infections such as influenza A virus. This response leads to the recruitment of innate and adaptive immune cells required for viral clearance. Some of these recruited cells remain within the lung following infection and contribute to enhanced viral control following subsequent infections. There is growing evidence that structural cells can also display long-term changes following infection or insults. Here we investigate long-term changes to mouse lung epithelial cells, fibroblasts, and endothelial cells following influenza virus infection and find that all three cell types maintain an imprint of the infection, particularly in genes associated with communication with T cells. Lung epithelial cells from IAV-infected mice display functional changes by more rapidly controlling influenza virus than cells from naive animals. This rapid anti-viral response and increased expression of molecules required to communicate with T cells demonstrates sustained and enhanced functions following infection. These data suggest lung structural cells could be effective targets for vaccines to boost durable protective immunity. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=135 SRC="FIGDIR/small/604410v5_ufig1.gif" ALT="Figure 1"> View larger version (55K): org.highwire.dtl.DTLVardef@2774e0org.highwire.dtl.DTLVardef@6a39e6org.highwire.dtl.DTLVardef@1ff5863org.highwire.dtl.DTLVardef@103df12_HPS_FORMAT_FIGEXP M_FIG C_FIG HighlightsO_LILung epithelial cells, fibroblasts, and blood endothelial cells maintain an inflammatory imprint of influenza A virus (IAV) infection for at least 40 days post-infection. C_LIO_LIIn vivo re-infection leads to a more spatially restricted anti-viral response compared to primary IAV-infected animals. C_LIO_LIT cells are not required for enhanced viral control early after re-infection in vivo C_LIO_LIEx vivo lung epithelial cells from IAV-infected mice more rapidly control IAV than cells from naive animals in the absence of immune cells. C_LI

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Worrell, J. C., Hargrave, K. E., Finney, G. E., Hansell, C., Cole, J. J., Singh Niijar, J., Morton, F. R., Pingen, M., Purnell, T., Mitchelson, K., Brennan, E., Allan, J., Ilia, G., Herder, V., Kennedy Dietrich, C., Doncheva, Y., Jamieson, N., MacLeod, M. K.. 2024-07-23. Lung structural cell dynamics are altered by influenza virus infection experience leading to rapid immune protection following viral re-challenge. https://doi.org/10.1101/2024.07.20.604410

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