Imp and Syp in vivo temporal RNA interactomes uncover networks of temporal regulators of Drosophila brain development.

Lee, J. Y.; Huang, N.; Samuels, T. J.; Davis, I.

doi:10.1101/2024.06.30.600407

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bioRxiv · 10.1101/2024.06.30.600407

Imp and Syp in vivo temporal RNA interactomes uncover networks of temporal regulators of Drosophila brain development.

Lee, J. Y.·Huang, N.·Samuels, T. J.·Davis, I.

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Abstract

Temporal patterning of neural progenitors is an evolutionarily conserved mechanism generating neural diversity. In Drosophila, post-embryonic neurogenesis requires the RNA-binding proteins (RBPs) Imp/IGF2BP and Syp/SYNCRIP. However, how they co-achieve their function is not well understood. Here, we elucidate the in vivo temporal RNA interactome landscapes of Imp and Syp during larval brain development. Imp and Syp bind a highly overlapping set of conserved mRNAs encoding proteins involved in neurodevelopment. We identify transcripts differentially occupied by Imp/Syp over time, featuring a network of known and novel candidate temporal regulators that are post-transcriptionally regulated by Imp/Syp. Furthermore, the physical and co-evolutionary relationships between Imp and Syp binding sites reveal a combinatorial, rather than competitive, mode of molecular interplay. Our study establishes a new in vivo framework for dissecting the temporal co-regulation of RBP networks as well as providing a resource for understanding neural fate specification.

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Lee, J. Y., Huang, N., Samuels, T. J., Davis, I.. 2024-06-30. Imp and Syp in vivo temporal RNA interactomes uncover networks of temporal regulators of Drosophila brain development.. https://doi.org/10.1101/2024.06.30.600407

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