Left-Right Brain-Wide Asymmetry of Neuroanatomy in the Mouse Brain

Silberfeld, A.; Roe, J. M.; Ellegood, J.; Lerch, J. P.; Qiu, L.; Kim, Y.; Lee, J. G.; Hopkins, W. D.; Grandjean, J.; Ou, Y.; Pourquie, O.

doi:10.1101/2024.06.25.600709

bioRxiv · 10.1101/2024.06.25.600709

Left-Right Brain-Wide Asymmetry of Neuroanatomy in the Mouse Brain

Silberfeld, A.·Roe, J. M.·Ellegood, J.·Lerch, J. P.·Qiu, L.·Kim, Y.·Lee, J. G.·Hopkins, W. D.·Grandjean, J.·Ou, Y.·Pourquie, O.

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Abstract

Left-right asymmetry of the human brain is widespread through its anatomy and function. However, limited microscopic understanding of it exists, particularly for anatomical asymmetry where there are few well-established animal models. In humans, most brain regions show subtle, population-average regional asymmetries in thickness or surface area, alongside a macro-scale twisting called the cerebral petalia in which the right hemisphere protrudes anteriorly past the left. Here, we ask whether neuroanatomical asymmetries can be observed in mice, leveraging 6 mouse neuroimaging cohorts from 5 different research groups ([~]3,500 animals). We found an anterior-posterior pattern of volume asymmetry with anterior regions larger on the right and posterior regions larger on the left. This pattern appears driven by similar trends in surface area and positional asymmetries, with the results together indicating a small brain-wide twisting pattern, similar to the human cerebral petalia. Furthermore, the results show no apparent relationship to known functional asymmetries in mice, emphasizing the complexity of the structure-function relationship in brain asymmetry. By establishing a signature of anatomical brain asymmetry in mice, we aim to provide a foundation for future studies to probe the mechanistic underpinnings of brain asymmetry seen in humans - a feature of the brain with extremely limited understanding. Significance StatementThe human brain shows significant left-right anatomical asymmetry. Understanding its microscopic basis has implications for studies of autism and schizophrenia, evolution, embryonic brain development, and the relationship between structure and function in the brain. One of the biggest challenges to understanding this aspect of the brain is that animal models are limited. Here we show a brain-wide twisting pattern of asymmetry in the mouse brain using over 3,500 animals from six independent cohorts. These findings provide a basis for using mice to interrogate the microscopic underpinnings of anatomical asymmetry in humans and a roadmap for exploring anatomical asymmetry in additional species.

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Silberfeld, A., Roe, J. M., Ellegood, J., Lerch, J. P., Qiu, L., Kim, Y., Lee, J. G., Hopkins, W. D., Grandjean, J., Ou, Y., Pourquie, O.. 2024-06-26. Left-Right Brain-Wide Asymmetry of Neuroanatomy in the Mouse Brain. https://doi.org/10.1101/2024.06.25.600709

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