Dopamine D1 receptor expression in dlPFC inhibitory parvalbumin neurons may contribute to higher visuospatial distractibility in marmosets versus macaques

Joyce, M. K. P.; Ivanov, T. G.; Krienen, F.; Mitchell, J.; Ma, S.; Inoue, W.; Nandy, A. P.; Datta, D.; Duque, A.; Arellano, J. I.; Gupta, R.; Gonzalez-Burgos, G.; Lewis, D. A.; Sestan, N.; McCarroll, S. A.; Martinez-Trujillo, J. C.; Froudist-Walsh, S.; Arnsten, A. F.

doi:10.1101/2024.06.15.599163

bioRxiv · 10.1101/2024.06.15.599163

Dopamine D1 receptor expression in dlPFC inhibitory parvalbumin neurons may contribute to higher visuospatial distractibility in marmosets versus macaques

Joyce, M. K. P.·Ivanov, T. G.·Krienen, F.·Mitchell, J.·Ma, S.·Inoue, W.·Nandy, A. P.·Datta, D.·Duque, A.·Arellano, J. I.·Gupta, R.·Gonzalez-Burgos, G.·Lewis, D. A.·Sestan, N.·McCarroll, S. A.·Martinez-Trujillo, J. C.·Froudist-Walsh, S.·Arnsten, A. F.

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Abstract

Marmosets and macaques are common non-human primate models of cognition, yet marmosets appear more distractible and perform worse in cognitive tasks. The dorsolateral prefrontal cortex (dlPFC) is pivotal for sustained attention, and prior macaque research suggests that dopaminergic modulation and inhibitory parvalbumin (PV) neurons could contribute to distractibility. Thus, we compared the two species using a visual fixation task with distractors, performed molecular and anatomical analyses in dlPFC, and linked functional microcircuitry with cognitive performance using computational modeling. We found that marmosets are more distractible than macaques, and that marmoset dlPFC PV neurons contain higher levels of dopamine-1 receptor (D1R) transcripts and protein, similar to their levels in mice. The modeling indicated that higher D1R expression in marmoset dlPFC PV neurons may increase distractibility by making dlPFC microcircuits more vulnerable to disruptions of their task-related persistent activity, especially when dopamine is released in dlPFC in response to unexpected salient stimuli. Declaration of InterestsThe authors have nothing to declare. Author ContributionsAFTA, MKPJ, TGI, and SFW designed the study; MKPJ collected and analyzed anatomical data; TGI designed the computational framework with guidance from RG and SFW; TGI performed the modeling simulations and data analysis; FM and SAM collected and analyzed transcriptomics data; GGB and DAL provided macaque brain tissue for transcriptomics performed by FM; SM and NS collected and analyzed phylogenetic transcriptomics dataset; JFM and ASN performed and analyzed the behavioral testing; IW and JMT provided the marmoset tissue for immunofluorescence; MKPJ, TGI, SFW, and AFTA wrote the first draft and all authors revised and edited subsequent drafts of the article.

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Joyce, M. K. P., Ivanov, T. G., Krienen, F., Mitchell, J., Ma, S., Inoue, W., Nandy, A. P., Datta, D., Duque, A., Arellano, J. I., Gupta, R., Gonzalez-Burgos, G., Lewis, D. A., Sestan, N., McCarroll, S. A., Martinez-Trujillo, J. C., Froudist-Walsh, S., Arnsten, A. F.. 2024-06-16. Dopamine D1 receptor expression in dlPFC inhibitory parvalbumin neurons may contribute to higher visuospatial distractibility in marmosets versus macaques. https://doi.org/10.1101/2024.06.15.599163

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