The impact of YabG mutations on C. difficile spore germination and processing of spore substrates

Osborne, M. S.; Brehm, J. N.; Olivenca, C.; Cochran, A. M.; Serrano, M.; Henriques, A. O.; Sorg, J. A.

doi:10.1101/2024.06.10.598338

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bioRxiv · 10.1101/2024.06.10.598338

The impact of YabG mutations on C. difficile spore germination and processing of spore substrates

Osborne, M. S.·Brehm, J. N.·Olivenca, C.·Cochran, A. M.·Serrano, M.·Henriques, A. O.·Sorg, J. A.

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Abstract

YabG is a sporulation-specific protease that is conserved among sporulating bacteria. C. difficile YabG processes cortex destined proteins preproSleC into proSleC and CspBA to CspB and CspA. YabG also affects synthesis of spore coat/exosporium proteins CotA and CdeM. In prior work that identified CspA as the co-germinant receptor, mutations in yabG were found which altered the co-germinants required to initiate spore germination. To understand how these mutations in the yabG locus contribute to C. difficile spore germination, we introduced these mutations into an isogenic background. Spores derived from C. difficile yabGC207A (catalytically inactive), C. difficile yabGA46D, C. difficile yabGG37E, and C. difficile yabGP153L strains germinated in response to TA alone. Recombinantly expressed and purified preproSleC incubated with E. coli lysate expressing wild type YabG resulted in the removal of the pre sequence from preproSleC. Interestingly, only YabGA46D showed any activity towards purified preproSleC. Mutation of the YabG processing site in preproSleC (R119A) led to YabG shifting its processing to R115 or R112. Finally, changes in yabG expression under the mutant promoters were analyzed using a SNAP-tag and revealed expression differences at early and late stages of sporulation. Overall, our results support and expand upon the hypothesis that YabG is important for germination and spore assembly and, upon mutation of the processing site, can shift where it cleaves substrates.

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Osborne, M. S., Brehm, J. N., Olivenca, C., Cochran, A. M., Serrano, M., Henriques, A. O., Sorg, J. A.. 2024-06-10. The impact of YabG mutations on C. difficile spore germination and processing of spore substrates. https://doi.org/10.1101/2024.06.10.598338

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