A phased chromosome-level genome assembly provides insights into the evolution of sex chromosomes in Amaranthus tuberculatus

Raiyemo, D. A.; Cutti, L.; Patterson, E. L.; Llaca, V.; Fengler, K.; Montgomery, J. S.; Morran, S.; Gaines, T. A.; Tranel, P. J.

doi:10.1101/2024.05.30.596720

bioRxiv · 10.1101/2024.05.30.596720

A phased chromosome-level genome assembly provides insights into the evolution of sex chromosomes in Amaranthus tuberculatus

Raiyemo, D. A.·Cutti, L.·Patterson, E. L.·Llaca, V.·Fengler, K.·Montgomery, J. S.·Morran, S.·Gaines, T. A.·Tranel, P. J.

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Abstract

O_LIAmaranthus tuberculatus (waterhemp) is a troublesome weed species of agronomic importance that is dioecious with an XY sex-determination system. The evolution of sex chromosomes, the contiguity of sex-determining region (SDR) and the expression pattern of genes within the SDR remain poorly understood. C_LIO_LIWe assembled the genome of a male A. tuberculatus, phased the genome into two chromosome-level haplotypes, and performed restriction site-associated DNA genome- wide association (RAD-GWA) analysis, comparative genomics, adaptive evolution analysis, and, with existing data, transcriptomic profiling to characterize the species sex chromosomes. C_LIO_LIComparative analysis enabled the identification of a [~]32.8 Mb SDR on chromosome 1 that is gene-poor, abundant in long terminal repeat (LTR) retrotransposons, and harbors two inversions. Synteny analysis revealed that chromosome 1 likely originated from the fusion of two ancestral chromosomes, and mRNA data indicated 93 genes out of the 531 protein-coding genes within the SDR of haplome 2 were differentially expressed between mature male and female flowers, with several of the genes enriched for Gene Ontology (GO) terms involved in floral development. C_LIO_LIBeyond adding to our understanding of sex chromosome evolution, the genomic resource provided here will be valuable for addressing further questions on adaptive trait evolution in Amaranthus. C_LI

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Raiyemo, D. A., Cutti, L., Patterson, E. L., Llaca, V., Fengler, K., Montgomery, J. S., Morran, S., Gaines, T. A., Tranel, P. J.. 2024-06-02. A phased chromosome-level genome assembly provides insights into the evolution of sex chromosomes in Amaranthus tuberculatus. https://doi.org/10.1101/2024.05.30.596720

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