Rationally Minimizing Natural Product Libraries Using Mass Spectrometry

Ness, M.; Peramuna, T.; Wendt, K. L.; Collins, J. E.; King, J. B.; Paes, R.; Santos, N. M.; Okeke, C.; Miller, C. R.; Chakrabarti, D.; Cichewicz, R. H.; McCall, L.-I.

doi:10.1101/2024.05.22.595232

bioRxiv · 10.1101/2024.05.22.595232

Rationally Minimizing Natural Product Libraries Using Mass Spectrometry

Ness, M.·Peramuna, T.·Wendt, K. L.·Collins, J. E.·King, J. B.·Paes, R.·Santos, N. M.·Okeke, C.·Miller, C. R.·Chakrabarti, D.·Cichewicz, R. H.·McCall, L.-I.

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Abstract

Natural product libraries are crucial to drug development, but large libraries drastically increase the time and cost during initial high throughput screens. Here, we developed a method that leverages liquid chromatography-tandem mass spectrometry spectral similarity to dramatically reduce library size, with minimal bioactive loss. This method offers a broadly applicable strategy for accelerated drug discovery with cost reductions, which enable implementation in resource-limited settings.

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Ness, M., Peramuna, T., Wendt, K. L., Collins, J. E., King, J. B., Paes, R., Santos, N. M., Okeke, C., Miller, C. R., Chakrabarti, D., Cichewicz, R. H., McCall, L.-I.. 2024-05-26. Rationally Minimizing Natural Product Libraries Using Mass Spectrometry. https://doi.org/10.1101/2024.05.22.595232

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