AARS Online: a collaborative database on the structure, function, and evolution of the aminoacyl-tRNA synthetases

Douglas, J.; Cui, H.; Perona, J. J.; Rodriguez, O. V.; Tyynismaa, H.; Alvarez-Carreno, C.; Ling, J.; Ribas-de-Pouplana, L.; Yang, X.-L.; Ibba, M.; Becker, H.; Fischer, F.; Sissler, M.; Carter, C. W.; Wills, P.

doi:10.1101/2024.05.15.594223

bioRxiv · 10.1101/2024.05.15.594223

AARS Online: a collaborative database on the structure, function, and evolution of the aminoacyl-tRNA synthetases

Douglas, J.·Cui, H.·Perona, J. J.·Rodriguez, O. V.·Tyynismaa, H.·Alvarez-Carreno, C.·Ling, J.·Ribas-de-Pouplana, L.·Yang, X.-L.·Ibba, M.·Becker, H.·Fischer, F.·Sissler, M.·Carter, C. W.·Wills, P.

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Abstract

The aminoacyl-tRNA synthetases (aaRS) are a large group of enzymes that implement the genetic code in all known biological systems. They attach amino acids to their cognate tRNAs, moonlight in various non-translational activities, and are linked to many genetic disorders. The aaRS have a subtle ontology characterized by structural and functional idiosyncrasies that vary from organism to organism, and protein to protein. Across the tree of life, the twenty-two coded amino acids are handled by sixteen evolutionary Families of Class I aaRS and twenty-one Families of Class II aaRS. We introduce AARS Online, an interactive Wikipedia-like tool curated by an international consortium of field experts. This platform systematizes existing knowledge about the aaRS by showcasing a taxonomically diverse selection of aaRS sequences and structures. Through its graphical user interface, AARS Online facilitates a seamless exploration between protein sequence and structure, providing a friendly introduction to the material for non-experts and a useful resource for experts. Curated multiple sequence alignments can be extracted for downstream analyses. Accessible at www.aars.online, AARS Online is a free resource to delve into the world of the aaRS.

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Douglas, J., Cui, H., Perona, J. J., Rodriguez, O. V., Tyynismaa, H., Alvarez-Carreno, C., Ling, J., Ribas-de-Pouplana, L., Yang, X.-L., Ibba, M., Becker, H., Fischer, F., Sissler, M., Carter, C. W., Wills, P.. 2024-05-15. AARS Online: a collaborative database on the structure, function, and evolution of the aminoacyl-tRNA synthetases. https://doi.org/10.1101/2024.05.15.594223

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