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bioRxiv · 10.1101/2024.05.14.594018

Structure-activity relationship of pyrrolidine pentamine derivatives as inhibitors of the aminoglycoside 6'-N-acetyltransferase type Ib

Abstract

Resistance to amikacin and other major aminoglycosides is commonly due to enzymatic acetylation by aminoglycoside 6'-N -acetyltransferase type I enzyme, of which type Ib [AAC(6')-Ib] is the most widespread among Gram-negative pathogens. Finding enzymatic inhibitors could be an effective way to overcome resistance and extend the useful life of amikacin. Small molecules possess multiple properties that make them attractive compounds to be developed as drugs. Mixture-based combinatorial libraries and positional scanning strategy led to the identification of a chemical scaffold, pyrrolidine pentamine, that, when substituted with the appropriate functionalities at five locations (R1 - R5), inhibits AAC(6')-Ib-mediated inactivation of amikacin. Structure-activity relationship (SAR) studies showed that while truncations to the molecule result in loss of inhibitory activity, modifications of functionalities and stereochemistry have different effects on the inhibitory properties. In this study, we show that alterations at position R1 of the two most active compounds, 2700.001 and 2700.003, reduced inhibition levels, demonstrating the essential nature not only of the presence of an S -phenyl moiety at this location but also the distance to the scaffold. On the other hand, modifications on the R3, R4, and R5 positions have varied effects, demonstrating the potential for optimization. A correlation analysis between molecular docking values ({Delta}G) and the dose required for two-fold potentiation of compounds described in this and the previous studies showed a significant correlation between {Delta}G values and inhibitory activity. HighlightsO_LIAmikacin resistance in Gram-negatives is mostly caused by the AAC(6')-Ib enzyme C_LIO_LIAAC(6')-Ib has been identified in most Gram-negative pathogens C_LIO_LIInhibitors of AAC(6')-Ib could be used to treat resistant infections C_LIO_LICombinatorial libraries and positional scanning identified an inhibitor C_LIO_LIThe lead compound can be optimized by structure activity relationship studies C_LI

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BibTeXRIS

Tran, T., Ramirez, M. S., Donow, H. M., Magana, A. J., LaVoi, T., Mamun, Y., Chapagain, P. P., Santos, R., Pinilla, C., Giulianotti, M. A., Tolmasky, M. E.. 2024-05-14. Structure-activity relationship of pyrrolidine pentamine derivatives as inhibitors of the aminoglycoside 6'-N-acetyltransferase type Ib. https://doi.org/10.1101/2024.05.14.594018

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