Proteasomal control of anti-CRISPRs for the regulation of CRISPR/Cas9 activity using Cas9-ACROBAT

Martin, T. D.; Watson, E. V.; Choi, M. Y.; Nabet, B.; Gray, N. S.; Xu, Q.; Elledge, S.

doi:10.1101/2024.05.13.593596

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bioRxiv · 10.1101/2024.05.13.593596

Proteasomal control of anti-CRISPRs for the regulation of CRISPR/Cas9 activity using Cas9-ACROBAT

Martin, T. D.·Watson, E. V.·Choi, M. Y.·Nabet, B.·Gray, N. S.·Xu, Q.·Elledge, S.

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Abstract

Small molecule-mediated proteasomal degradation of proteins is a powerful tool for synthetic regulation of biological activity. To control Cas9 activity in cells, we engineered an anti-CRISPR protein, AcrIIA4, fused to a degradation (dTAG) or small molecule assisted shutoff (SMASh) tag. Co-expression of the tagged AcrIIA4 along with Cas9 and riboswitch-regulated sgRNAs enables precise tunable control of CRISPR activity by small molecule addition.

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Martin, T. D., Watson, E. V., Choi, M. Y., Nabet, B., Gray, N. S., Xu, Q., Elledge, S.. 2024-05-13. Proteasomal control of anti-CRISPRs for the regulation of CRISPR/Cas9 activity using Cas9-ACROBAT. https://doi.org/10.1101/2024.05.13.593596

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