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bioRxiv · 10.1101/2024.05.10.593571

Associations Between Prenatal Vitamin D and Placental Gene Expression

Parenti, M.·Melough, M. M.·Lapehn, S.·MacDonald, J.·Firsick, E. J.·Choi, H. Y.·Derefinko, K. J.·Enquobahrie, D. A.·Carroll, K. N.·LeWinn, K. Z.·Bush, N. R.·Zhao, Q.·Sathyanarayana, S.·Paquette, A. G.
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Abstract

BackgroundVitamin D is a hormone regulating gene transcription. Prenatal vitamin D has been linked to immune and vascular function in the placenta, a key organ of pregnancy. To date, studies of vitamin D and placental gene expression have focused on a limited number of candidate genes. Transcriptome-wide RNA sequencing can provide a more complete representation of the placental effects of vitamin D. ObjectiveWe investigated the association between prenatal vitamin D levels and placental gene expression in a large, prospective pregnancy cohort. MethodsParticipants were recruited in Shelby County, Tennessee in the Conditions Affecting Neurocognitive Development and Learning in Early childhood (CANDLE) study. Vitamin D level (plasma total 25-hydroxyvitatmin D, [25(OH)D]) was measured at mid-pregnancy (16-28 weeks gestation) and delivery. Placenta samples were collected at birth. RNA was isolated and sequenced. We identified differentially expressed genes (DEGs) using adjusted linear regression models. We also conducted weighted gene co-expression network analysis (WGCNA). ResultsThe median 25(OH)D of participants was 21.8 ng/mL at mid-pregnancy (N=774, IQR: 15.4-26.5 ng/mL) and 23.6 ng/mL at delivery (N=753, IQR: 16.8-29.1 ng/mL). Placental expression of 25 DEGs was associated with 25(OH)D at mid-pregnancy, but no DEG was associated with 25(OH)D at delivery. DEGs were related to energy metabolism, cytoskeletal function, and RNA transcription. Using WGCNA, we identified 2 gene modules whose expression was associated with 25(OH)D at mid-pregnancy and 1 module associated with 25(OH)D at delivery. These modules were enriched for genes related to mitochondrial and cytoskeletal function, and were regulated by transcription factors including ARNT2, BHLHE40, FOSL2, JUND, and NFKB1. ConclusionsOur results indicate that 25(OH)D during mid-pregnancy, but not at delivery, is associated with placental gene expression at birth. Future research is needed to investigate a potential role of vitamin D in programming placental mitochondrial metabolism, intracellular transport, and transcriptional regulation during pregnancy.

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Parenti, M., Melough, M. M., Lapehn, S., MacDonald, J., Firsick, E. J., Choi, H. Y., Derefinko, K. J., Enquobahrie, D. A., Carroll, K. N., LeWinn, K. Z., Bush, N. R., Zhao, Q., Sathyanarayana, S., Paquette, A. G.. 2024-05-12. Associations Between Prenatal Vitamin D and Placental Gene Expression. https://doi.org/10.1101/2024.05.10.593571

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