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bioRxiv · 10.1101/2024.05.08.593103

Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope

Loretan, M.·Barella, M.·Fuchs, N.·Kocabey, S.·Kolataj, K.·Stefani, F. D.·Acuna, G. P.
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Abstract

We present a novel, low-cost, portable smartphone-based fluorescence microscope capable of directly detecting single molecules without signal amplification. The setup leverages the image sensors and data handling capacity of mass-produced smartphones, making it adaptable to any smartphone and capable of detecting single molecules across the visible spectral range. We showcase this capability through single-molecule measurements on DNA origami models and super-resolution microscopy of biological cells by single-molecule localization microscopy. This development paves the way for biotechnology innovations making use of massively distributed or personalized assays with single-molecule sensitivity with the potential to revolutionize digital bioassays, point-of-care diagnostics, field expeditions, STEM outreach, and life science education.

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Loretan, M., Barella, M., Fuchs, N., Kocabey, S., Kolataj, K., Stefani, F. D., Acuna, G. P.. 2024-05-10. Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope. https://doi.org/10.1101/2024.05.08.593103

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