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bioRxiv · 10.1101/2024.04.24.590988

Antibody-free time-resolved terbium luminescence assays designed for cyclin-dependent kinase 5 (CDK5)

Heier, J. L.·Boselli, D. J.·Parker, L. L.
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Abstract

Novel time-resolved terbium luminescence assays were developed for CDK5 and CDK2 by designing synthetic substrates which incorporate phospho-inducible terbium sensitizing motifs with kinase substrate consensus sequences. Substrates designed for CDK5 showed no phosphorylation by CDK2, opening the possibility for CDK5-specific assay development for selective drug discovery.

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Heier, J. L., Boselli, D. J., Parker, L. L.. 2024-04-24. Antibody-free time-resolved terbium luminescence assays designed for cyclin-dependent kinase 5 (CDK5). https://doi.org/10.1101/2024.04.24.590988

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