Ca2+-dependent phosphodiesterase 1 regulates the plasticity of striatal spiny projection neuron glutamatergic synapses

Zhai, S.; Otsuka, S.; Xu, J.; Clarke, V. R. J.; Tkatch, T.; Wokosin, D.; Xie, Z.; Tanimura, A.; Agarwal, H. K.; Ellis-Davies, G. C. R.; Contractor, A.; Surmeier, D. J.

doi:10.1101/2024.04.24.590962

bioRxiv · 10.1101/2024.04.24.590962

Ca2+-dependent phosphodiesterase 1 regulates the plasticity of striatal spiny projection neuron glutamatergic synapses

Zhai, S.·Otsuka, S.·Xu, J.·Clarke, V. R. J.·Tkatch, T.·Wokosin, D.·Xie, Z.·Tanimura, A.·Agarwal, H. K.·Ellis-Davies, G. C. R.·Contractor, A.·Surmeier, D. J.

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Abstract

Long-term synaptic plasticity at glutamatergic synapses on striatal spiny projection neurons (SPNs) is central to learning goal-directed behaviors and habits. Although considerable attention has been paid to the mechanisms underlying synaptic strengthening and new learning, little scrutiny has been given to those involved in the attenuation of synaptic strength that attends suppression of a previously learned association. Our studies revealed a novel, non-Hebbian, long-term, postsynaptic depression of glutamatergic SPN synapses induced by interneuronal nitric oxide (NO) signaling (NO-LTD) that was preferentially engaged at quiescent synapses. This form of plasticity was gated by local Ca2+ influx through CaV1.3 Ca2+ channels and stimulation of phosphodiesterase 1 (PDE1), which degraded cyclic guanosine monophosphate (cGMP) and blunted NO signaling. Consistent with this model, mice harboring a gain-of-function mutation in the gene coding for the pore-forming subunit of CaV1.3 channels had elevated depolarization-induced dendritic Ca2+ entry and impaired NO-LTD. Extracellular uncaging of glutamate and intracellular uncaging of cGMP suggested that this Ca2+-dependent regulation of PDE1 activity allowed for local regulation of dendritic NO signaling. This inference was supported by simulation of SPN dendritic integration, which revealed that dendritic spikes engaged PDE1 in a branch-specific manner. In a mouse model of Parkinsons disease (PD), NO-LTD was absent not because of a postsynaptic deficit in NO signaling machinery, but rather due to impaired interneuronal NO release. Re-balancing intrastriatal neuromodulatory signaling in the PD model restored NO release and NO-LTD. Taken together, these studies provide novel insights into the mechanisms governing NO-LTD in SPN and its role in psychomotor disorders, like PD.

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Zhai, S., Otsuka, S., Xu, J., Clarke, V. R. J., Tkatch, T., Wokosin, D., Xie, Z., Tanimura, A., Agarwal, H. K., Ellis-Davies, G. C. R., Contractor, A., Surmeier, D. J.. 2024-04-25. Ca2+-dependent phosphodiesterase 1 regulates the plasticity of striatal spiny projection neuron glutamatergic synapses. https://doi.org/10.1101/2024.04.24.590962

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