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bioRxiv · 10.1101/2024.04.19.590246

Flumequine, a fluoroquinolone in disguise

Swinkels, A.·Fischer, E.·Korving, L.·Chirstodoulou, R.·Wagenaar, J. A.·Zomer, A. L.
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Abstract

Fluoroquinolone resistance in E. coli isolates from livestock in Europe remains high even though the EMA restricted fluoroquinolone use in animals. However, flumequine, a quinolone similar to fluoroquinolones, is still used in livestock in the Netherland, Belgium, Greece or France. Therefore, we investigated whether flumequine selects for the same resistance mechanisms in E. coli. To accomplish this, we investigated and enumerated resistant and non-resistant E. coli isolates obtained from caecal fermentation assays and from broilers exposed to different concentrations of flumequine and enrofloxacin. Flumequine usage leads to approximately 3-fold increase of resistant E. coli in the caecal fermentation, comparable with enrofloxacin. After in vitro exposure to flumequine and enrofloxacin we detected the same SNPs (S83L, D87G) in gyrA. Furthermore, the same resistance-causing SNPs were found in phenotypic resistant E. coli isolates from broilers treated with enrofloxacin and flumequine. Flumequine induces similar resistance mechanisms as enrofloxacin and restriction for use should be the same.

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Swinkels, A., Fischer, E., Korving, L., Chirstodoulou, R., Wagenaar, J. A., Zomer, A. L.. 2024-04-19. Flumequine, a fluoroquinolone in disguise. https://doi.org/10.1101/2024.04.19.590246

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