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bioRxiv · 10.1101/2024.04.18.590060

nP-collabs: Investigating counterion mediated bridges in the multiply phosphorylated tau-R2 repeat

Marien, J.·Prevost, C.·Sacquin-Mora, S.
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Abstract

Tau is an instrinsically disordered (IDP), microtubule-associated protein (MAP) that plays a key part in microtubule assembly and organization. The function of tau can be regulated via multiple phosphorylation sites. These post-translational modifications are known to decrease the binding affinity of tau for microtubules, and abnormal tau phosphorylation patterns are involved in Alzheimers disease. Using all-atom molecular dynamics (MD) simulations, we compared the conformational landscapes explored by the tau R2 repeat domain (which comprises a strong tubulin binding site) in its native state and with multiple phosphorylations on the S285, S289 and S293 residues, with four different standard force field (FF)/water model combinations. We find that the different parameters used for the phosphate groups (which can be more or less flexible) in these FFs, and the specific interactions between bulk cations and water lead to the formation of a specific type of counterion bridge, termed nP-collab (for nPhosphate collaboration, with n being an integer), where counterions form stable structures binding with two or three phosphate groups simultaneously. The resulting effect of nP-collabs on the tau-R2 conformational space differs when using sodium or potassium cations, and is likely to impact the peptide overall dynamics, and how this MAP interacts with tubulins. We also investigated the effect of phosphoresidues spacing and ionic concentration by modeling polyalanine peptides containing two phosphoserines located one to six residues apart. Three new metrics specifically tailored for IDPs (Proteic Menger Curvature, Local Curvature and Local Flexibility) were introduced, which allow us to fully characterize the impact of nP-collabs on the dynamics of disordered peptides at the residue level. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=106 SRC="FIGDIR/small/590060v2_ufig1.gif" ALT="Figure 1"> View larger version (20K): org.highwire.dtl.DTLVardef@1b1b599org.highwire.dtl.DTLVardef@99047org.highwire.dtl.DTLVardef@1df34b9org.highwire.dtl.DTLVardef@fe60b2_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Marien, J., Prevost, C., Sacquin-Mora, S.. 2024-04-18. nP-collabs: Investigating counterion mediated bridges in the multiply phosphorylated tau-R2 repeat. https://doi.org/10.1101/2024.04.18.590060

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