Associations between SARS-CoV-2 Infection or COVID-19 Vaccination and Human Milk Composition: A Multi-Omics Approach

Couvillion, S. P.; Nakayasu, E. S.; Webb-Robertson, B.-J.; Yang, I.; Eder, J.; Nicora, C. D.; Bramer, L. M.; Gao, Y.; Fox, A.; DeCarlo, C.; Yang, X.; Zhou, M.; Pace, R. M.; Williams, J. E.; McGuire, M. A.; McGuire, M.; Metz, T.; Powell, R. L. R.

doi:10.1101/2024.04.12.589299

bioRxiv · 10.1101/2024.04.12.589299

Associations between SARS-CoV-2 Infection or COVID-19 Vaccination and Human Milk Composition: A Multi-Omics Approach

Couvillion, S. P.·Nakayasu, E. S.·Webb-Robertson, B.-J.·Yang, I.·Eder, J.·Nicora, C. D.·Bramer, L. M.·Gao, Y.·Fox, A.·DeCarlo, C.·Yang, X.·Zhou, M.·Pace, R. M.·Williams, J. E.·McGuire, M. A.·McGuire, M.·Metz, T.·Powell, R. L. R.

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Abstract

The risk of contracting SARS-CoV-2 via human milk-feeding is virtually non-existent. Adverse effects of COVID-19 vaccination for lactating individuals are not different from the general population, and no evidence has been found that their infants exhibit adverse effects. Yet, there remains substantial hesitation among this population globally regarding the safety of these vaccines. Herein we aimed to determine if compositional changes in milk occur following infection or vaccination, including any evidence of vaccine components. Using an extensive multi-omics approach, we found that compared to unvaccinated individuals SARS-CoV-2 infection was associated with significant compositional differences in 67 proteins, 385 lipids, and 13 metabolites. In contrast, COVID-19 vaccination was not associated with any changes in lipids or metabolites, although it was associated with changes in 13 or fewer proteins. Compositional changes in milk differed by vaccine. Changes following vaccination were greatest after 1-6 hours for the mRNA-based Moderna vaccine (8 changed proteins), 3 days for the mRNA-based Pfizer (4 changed proteins), and adenovirus-based Johnson and Johnson (13 changed proteins) vaccines. Proteins that changed after both natural infection and Johnson and Johnson vaccine were associated mainly with systemic inflammatory responses. In addition, no vaccine components were detected in any milk sample. Together, our data provide evidence of only minimal changes in milk composition due to COVID-19 vaccination, with much greater changes after natural SARS-CoV-2 infection. IMPORTANCEThe impact of the observed changes in global milk composition on infant health remain unknown. These findings emphasize the importance of vaccinating the lactating population against COVID-19, as compositional changes in milk were found to be far less evident after vaccination compared to SARS-CoV-2 infection. Importantly, vaccine components were not detected in milk after vaccination.

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Couvillion, S. P., Nakayasu, E. S., Webb-Robertson, B.-J., Yang, I., Eder, J., Nicora, C. D., Bramer, L. M., Gao, Y., Fox, A., DeCarlo, C., Yang, X., Zhou, M., Pace, R. M., Williams, J. E., McGuire, M. A., McGuire, M., Metz, T., Powell, R. L. R.. 2024-04-15. Associations between SARS-CoV-2 Infection or COVID-19 Vaccination and Human Milk Composition: A Multi-Omics Approach. https://doi.org/10.1101/2024.04.12.589299

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