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bioRxiv · 10.1101/2024.04.12.588853

SleepInvestigatoR: A flexible R function for analyzing scored sleep in rodents

Gamble, M. C.·Williams, B. R.·McKenna, J. T.·Logan, R. W.
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Abstract

Analyzing scored sleep is a fundamental prerequisite to understanding how sleep changes between health and disease. Classically, this is accomplished by manually calculating various measures (e.g., percent of non-rapid eye movement sleep) from a collection of scored sleep files. This process can be tedious and error prone especially when studies include a large number of animals or involve long recording sessions. To address this issue, we present SleepInvestigatoR, a versatile tool that can quickly organize and analyze multiple scored sleep files into a single output. The function is written in the open-source statistical language R and has a total of 25 parameters that can be set to match a wide variety of experimenter needs. SleepInvestigatoR delivers a total of 22 unique measures of sleep, including all measures commonly reported in the rodent literature. A simple plotting function is also provided to quickly graph and visualize the scored data. All code is designed to be implemented with little formal coding knowledge and step-by-step instructions are provided on the corresponding GitHub page. Overall, SleepInvestigatoR provides the sleep researcher a critical tool to increase efficiency, interpretation, and reproducibility in analyzing scored rodent sleep.

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Gamble, M. C., Williams, B. R., McKenna, J. T., Logan, R. W.. 2024-04-15. SleepInvestigatoR: A flexible R function for analyzing scored sleep in rodents. https://doi.org/10.1101/2024.04.12.588853

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