The longitudinal behavioral effects of acute exposure to galactic cosmic radiation in female C57BL/6J mice: implications for deep space missions, female crews, and potential antioxidant countermeasures

Yun, S.; Kiffer, F. C.; Guzman, C. S.; Soler, I.; Bancroft, G. L.; Haas, H. A.; Shi, R.; Patel, R. A.; Lara-Jimenez, J. A.; Kumar, P. L.; Tran, F. H.; Ahn, K. J.; Rong, Y.; Luitel, K.; Shay, J. W.; Eisch, A. J.

doi:10.1101/2024.04.12.588768

bioRxiv · 10.1101/2024.04.12.588768

The longitudinal behavioral effects of acute exposure to galactic cosmic radiation in female C57BL/6J mice: implications for deep space missions, female crews, and potential antioxidant countermeasures

Yun, S.·Kiffer, F. C.·Guzman, C. S.·Soler, I.·Bancroft, G. L.·Haas, H. A.·Shi, R.·Patel, R. A.·Lara-Jimenez, J. A.·Kumar, P. L.·Tran, F. H.·Ahn, K. J.·Rong, Y.·Luitel, K.·Shay, J. W.·Eisch, A. J.

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Abstract

Galactic cosmic radiation (GCR) is an unavoidable risk to astronauts that may affect mission success. Male rodents exposed to 33-beam-GCR (33-GCR) show short-term cognitive deficits but reports on female rodents and long-term assessment is lacking. Here we asked: What are the longitudinal behavioral effects of 33-GCR on female mice? Also, can an antioxidant/anti-inflammatory compound mitigate the impact of 33-GCR? Mature (6-month-old) C57BL/6J female mice received the antioxidant CDDO-EA (400 {micro}g/g of food) or a control diet (vehicle, Veh) for 5 days and either Sham-irradiation (IRR) or whole-body 33-GCR (0.75Gy) on the 4th day. Three-months post-IRR, mice underwent two touchscreen-platform tests: 1) location discrimination reversal (which tests behavior pattern separation and cognitive flexibility, two abilities reliant on the dentate gyrus) and 2) stimulus-response learning/extinction. Mice then underwent arena-based behavior tests (e.g. open field, 3-chamber social interaction). At the experiment end (14.25-month post-IRR), neurogenesis was assessed (doublecortin-immunoreactive [DCX+] dentate gyrus neurons). Female mice exposed to Veh/Sham vs. Veh/33-GCR had similar pattern separation (% correct to 1st reversal). There were two effects of diet: CDDO-EA/Sham and CDDO-EA/33-GCR mice had better pattern separation vs. their respective control groups (Veh/Sham, Veh/33-GCR), and CDDO-EA/33-GCR mice had better cognitive flexibility (reversal number) vs. Veh/33-GCR mice. Notably, one radiation effect/CDDO-EA countereffect also emerged: Veh/33-GCR mice had worse stimulus-response learning (days to completion) vs. all other groups, including CDDO-EA/33-GCR mice. In general, all mice show normal anxiety-like behavior, exploration, and habituation to novel environments. There was also a change in neurogenesis: Veh/33-GCR mice had fewer DCX+ dentate gyrus immature neurons vs. Veh/Sham mice. Our study implies space radiation is a risk to a female crews longitudinal mission-relevant cognitive processes and CDDO-EA is a potential dietary countermeasure for space-radiation CNS risks.

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Yun, S., Kiffer, F. C., Guzman, C. S., Soler, I., Bancroft, G. L., Haas, H. A., Shi, R., Patel, R. A., Lara-Jimenez, J. A., Kumar, P. L., Tran, F. H., Ahn, K. J., Rong, Y., Luitel, K., Shay, J. W., Eisch, A. J.. 2024-04-15. The longitudinal behavioral effects of acute exposure to galactic cosmic radiation in female C57BL/6J mice: implications for deep space missions, female crews, and potential antioxidant countermeasures. https://doi.org/10.1101/2024.04.12.588768

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