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bioRxiv · 10.1101/2024.04.11.589086

A Novel Simple ImmunoAssay for Quantification of Blood Anti-NMDAR1 Autoantibodies

Vaughn, M. N.·Powell, S.·Risbrough, V.·Zhou, X.
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Abstract

High titers of anti-NMDAR1 autoantibodies in human brain cause anti-NMDAR1 encephalitis, a rare disease that displays a variety of psychiatric symptoms and neurological symptoms. Currently, immunohistochemical staining and cell-based assays are the standard methods for detection and semi-quantification of the anti-NMDAR1 autoantibodies. Low titers of blood circulating anti-NMDAR1 autoantibodies have been reported in a significant subset of the general human population. However, detection and quantification of these low titers of blood circulating anti-NMDAR1 autoantibodies are problematic because of high non-specific background from less diluted serum/plasma. Development of a new method to quantify these low titers of blood anti-NMDAR1 autoantibodies is necessary to understand their potential impacts on psychiatric symptoms and cognition. Based on our previous One-Step assay, we report the development of a novel simple immunoassay to quantify cross-species blood anti-NMDAR1 autoantibodies, and its validation with immunohistochemistry and cell-based assays in both humans and mice.

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Vaughn, M. N., Powell, S., Risbrough, V., Zhou, X.. 2024-04-15. A Novel Simple ImmunoAssay for Quantification of Blood Anti-NMDAR1 Autoantibodies. https://doi.org/10.1101/2024.04.11.589086

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